AKT2 antibody

Catalog No. ABIN7647941

The Mouse Monoclonal anti-AKT2 antibody has been validated for WB, IHC, ICC and IP. It is suitable to detect AKT2 in samples from Human.

Quick Overview for AKT2 antibody (ABIN7647941)

Target: AKT2 (V-Akt Murine Thymoma Viral Oncogene Homolog 2 (AKT2))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This AKT2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP)

Product Details anti-AKT2 Antibody

Purpose: Monoclonal Antibody to Protein Kinase B Beta (PKBb)

Specificity: The antibody is a mouse monoclonal antibody raised against PKBb. It has been selected for its ability to recognize PKBb in immunohistochemical staining and western blotting.

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Application Details

Application Notes: Western blotting: 0.2-2 μg/mL,1:500-5000 Immunohistochemistry: 5-20 μg/mL,1:50-200 Immunocytochemistry: 5-20 μg/mL,1:50-200 Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS, pH 7.4, containing 0.02 % Sodium azide, 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Target Details for AKT2

Target: AKT2 (V-Akt Murine Thymoma Viral Oncogene Homolog 2 (AKT2))

Alternative Name: PKBb

Background: AKT2, PKBBETA, PRKB-B, RAC-BETA, RAC-Beta Serine/Threonine-Protein Kinase, V-Akt Murine Thymoma Viral Oncogene Homolog 2

UniProt: P31751

Pathways: PI3K-Akt Signaling, RTK Signaling, AMPK Signaling, TLR Signaling, Cellular Glucan Metabolic Process, Regulation of Carbohydrate Metabolic Process, Hepatitis C, VEGF Signaling