ARF1 antibody (C-Term)

Catalog No. ABIN7649207

The Mouse Monoclonal anti-ARF1 antibody has been validated for WB and IF. It is suitable to detect ARF1 in samples from Human, Rat and Mouse.

Quick Overview for ARF1 antibody (C-Term) (ABIN7649207)

Target: ARF1 (ADP-Ribosylation Factor 1 (ARF1))

Reactivity: Human, Rat, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This ARF1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF)

Clone: 3F1

Product Details anti-ARF1 Antibody

Binding Specificity: AA 174-180, C-Term

Purpose: ARF1 (Golgi Apparatus Marker)(3F1)

Characteristics: The ADP-ribosylation factor (ARF) family comprises a group of structurally and functionally conserved proteins, which are members of the Ras superfamily of regulatory GTP-binding proteins. The ARF family is divided functionally into the ARF and the ARF-like proteins. ARF's share more than 60 % sequence identity, appear to be ubiquitous in eukaryotes, and are highly conserved evolutionarily. ARF is involved in intracellular protein traffic to and within the Golgi complex. ARF has a number of disparate activities including maintenance of organelle integrity, assembly of coat proteins, as a co-factor for cholera toxin and as an activator of phospholipase D. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Synthetic peptide SNQLRNQ, corresponding to C terminal amino acids 174-180 of Human ARF1.

Isotype: IgG2a, kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Positive Control: HeLa cells. Kidney, Liver, Brain or Breast.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: PBS, 0.05 % BSA, 0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment:

Stable at room temperature or 37°C for 7 days.

Store at 2 to 8°C. Protect fluorescent conjugates from light

Expiry Date: 24 months

Target Details for ARF1

Target: ARF1 (ADP-Ribosylation Factor 1 (ARF1))

Alternative Name: ADP-Ribosylation Factor 1

Background:

Synonyms: ADP-ribosylation factor 1, ARF1

Gene Symbol: ARF1

Molecular Weight: 20 kDa

Gene ID: 375

UniProt: P84077

Pathways: Transition Metal Ion Homeostasis, Inositol Metabolic Process