BRCA1 antibody (AA 445-620) (CF®740)

Catalog No. ABIN7650146

The Mouse Monoclonal anti-BRCA1 antibody has been validated for IHC and IHC (fp). It is suitable to detect BRCA1 in samples from Human.

Quick Overview for BRCA1 antibody (AA 445-620) (CF®740) (ABIN7650146)

Target: BRCA1 (Breast Cancer 1 (BRCA1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This BRCA1 antibody is conjugated to CF®740

Application: Immunohistochemistry (IHC), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Clone: BRCA1-1398

Product Details anti-BRCA1 Antibody (CF®740)

Binding Specificity: AA 445-620

Purpose: BRCA1 (Breast Marker) (BRCA1/1398), CF740 conjugate

Characteristics: BRCA1 is a nuclear phosphoprotein that plays a role in maintaining genomic stability, and it also acts as a tumor suppressor. It combines with other tumor suppressors, DNA damage sensors, and signal transducers to form a large multi-subunit protein complex known as the BRCA1-associated genome surveillance complex (BASC). BRCA1 associates with RNA polymerase II, and through the C-terminal domain, also interacts with histone deacetylase complexes. This protein thus plays a role in transcription, DNA repair of double-stranded breaks, and recombination. Mutations in this gene are responsible for approximately 40 % of inherited breast cancers and more than 80 % of inherited breast and ovarian cancers. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant human BRCA1 protein fragment (aa445-620)

Isotype: IgG1

Application Details

Application Notes: Immunohistology Formalin-fixed 5-10 μg/mL for 60 min at RT. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM citrate buffer pH 6.0for 10-20 min followed by cooling at RT for 20 min. Flow Cytometry 0.5-1 μg/million cells/0.1 mL. Immunofluorescence 1-2 μg/mL. Optimal dilution for a specific application should be determined by user

Comment:

Positive Control: A431 or HeLa Cells. Breast, Ovarian or Renal Cell Carcinoma

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % rBSA, 0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Storage: 4 °C

Storage Comment:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Expiry Date: 24 months

Target Details for BRCA1

Target: BRCA1 (Breast Cancer 1 (BRCA1))

Alternative Name: BRCA1

Background:

Synonyms: BRCA1, Breast and ovarian cancer susceptibility protein 1, Early Onset Breast Cancer 1, Protein phosphatase 1 regulatory subunit 53, RING finger protein 53

Gene Symbol: BRCA1

Molecular Weight: 220 kDa

Gene ID: 672

UniProt: P38398

Pathways: Cell Division Cycle, DNA Damage Repair, Intracellular Steroid Hormone Receptor Signaling Pathway, Positive Regulation of Response to DNA Damage Stimulus