MERTK antibody (AA 55-148) (CF®740)

Catalog No. ABIN7650176

The Mouse Monoclonal anti-MERTK antibody has been validated for IHC and IHC (fp). It is suitable to detect MERTK in samples from Human.

Quick Overview for MERTK antibody (AA 55-148) (CF®740) (ABIN7650176)

Target: MERTK (C-Mer Proto-Oncogene Tyrosine Kinase (MERTK))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This MERTK antibody is conjugated to CF®740

Application: Immunohistochemistry (IHC), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Clone: MERTK-3015

Product Details anti-MERTK Antibody (CF®740)

Binding Specificity: AA 55-148

Purpose: MerTK (Innate Immune Checkpoint)(MERTK/3015), CF740 conjugate

Characteristics: MerTK, also called c-Mer, is a member of the Mer/Axl/Tyro3 receptor kinase family. It is a 984 residue transmembrane protein made up of one tyrosine kinase domain, two Fibronectin type-III domains and two immunoglobulinlike C2-type domains. MerTK is the mammalian ortholog of the chicken retroviral oncogene product v-Eyk. This protein plays a critical role in macrophage activation, platelet aggregation, clot stability and the efficient removal of apoptotic cells. Specifically, MerTK acts as a signaling molecule, triggering outer segment ingestion in the retinal pigment epithelium (RPE) phagocytic process. Evidence suggests that MerTK signals via interaction with phosphatidylinositol-specific phospholipase C 2). When the gene encoding for MerTK is mutated, the RPE phagocytosis pathway is disrupted and autosomal recessive retinitis pigmentosa (RP) may result, leading to degeneration of retinal photoreceptor cells. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant human MERTK protein fragment (around aa 55-148)

Isotype: IgG

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Positive Control: Human colon tissue (IHC).

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % rBSA, 0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Storage: 4 °C

Storage Comment:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Expiry Date: 24 months

Target Details for MERTK

Target: MERTK (C-Mer Proto-Oncogene Tyrosine Kinase (MERTK))

Alternative Name: MerTK

Background:

Synonyms: cEyk, MER receptor tyrosine kinase, MERK, MERPEN, nmf12, Nyk, Proto-oncogene c-Mer, RP38, STK kinase

Gene Symbol: MERTK

Molecular Weight: ~110 kDa

Gene ID: 10461

UniProt: Q12866

Pathways: RTK Signaling