Calnexin antibody (N-Term) (CF®740)

Catalog No. ABIN7650425

The Mouse Monoclonal anti-Calnexin antibody has been validated for WB, IHC, IF, FACS and IHC (fp). It is suitable to detect Calnexin in samples from Human.

Quick Overview for Calnexin antibody (N-Term) (CF®740) (ABIN7650425)

Target: Calnexin (CANX)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Calnexin antibody is conjugated to CF®740

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Clone: CANX-1541

Product Details anti-Calnexin Antibody (CF®740)

Binding Specificity: N-Term

Purpose: Calnexin (Endoplasmic Reticulum Marker) (CANX/1541), CF740 conjugate

Characteristics: Calnexin is a type-I integral membrane protein of the endoplasmic reticulum (ER). It is a calcium-binding chaperone protein that interacts transiently with newly synthesized N-linked glycoproteins, facilitating protein folding and assembly. It may also play a central role in the quality control of protein folding by retaining incorrectly folded protein subunits within the ER for degradation. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant N-terminal fragment of human Calnexin protein

Isotype: IgG2b, kappa

Application Details

Application Notes: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Immunofluorescence: 0.5-1 μg/mL. Immunohistology (formalin) 1-2 μg/mL. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM Tris, 1 mM EDTA pH 9.0 for 10-20 min followed by cooling at RT for 20 min. Flow Cytometry 0.5-1 μg/million cells/0.1 mL. Western blotting 0.5-1 μg/mL. Optimal dilution for a specific application should be determined by user

Comment:

Positive Control: HeLa, MCF-7 or U2OS cells. Kidney or small intestine.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % rBSA, 0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Storage: 4 °C

Storage Comment:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Expiry Date: 24 months

Target Details for Calnexin

Target: Calnexin (CANX)

Alternative Name: Calnexin

Background:

Synonyms: Calnexin, CANX, IP90, Major histocompatibility complex class I antigen-binding protein p88

Gene Symbol: CANX

Molecular Weight: 67 kDa (predicted), 80-90 kDa (observed)

Gene ID: 821

UniProt: P27824

Pathways: MAPK Signaling, Thyroid Hormone Synthesis