Calnexin antibody (ExoBrite™ 800)

Catalog No. ABIN7650432

The ExoBrite™ 800-conjugated Mouse Monoclonal anti-Calnexin antibody (ABIN7650432) specifically detects Calnexin in WB and ExoS. The antibody is reactive with Human samples.

Quick Overview for Calnexin antibody (ExoBrite™ 800) (ABIN7650432)

Target: Calnexin (CANX)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Calnexin antibody is conjugated to ExoBrite™ 800

Application: Western Blotting (WB), Exosome Staining (ExoS)

Product Details anti-Calnexin Antibody (ExoBrite™ 800)

Purpose: ExoBrite™ 770/800 Calnexin Western Antibody (100 tests)

Characteristics: ExoBrite™ 770/800 Calnexin Western Antibody is designed to detect the ER marker calnexin by near-IR WB as a control for cellular contamination of exosome preps. The conjugate has Ex/Em at 770/797 nm for detection in the 800 nm channel. Supplied as 1 mL of 100 μg/mL for 100 mini-blots.

Isotype: IgG1, kappa

Application Details

Application Notes:

Suggested Antibody Volume per Assay: 10 uL/test

Product features: Designed for western blot detection, validated with cell and exosome lysates

Application Notes: Recommended amount for western blot: 100 ng/mL = 1:1000 dilution. Optimal concentration to be determined by end-user.

Comment:

Positive Control: MCF-7 cells | MCF-7 derived exosomes

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 100 μg/mL

Buffer: Proprietary buffer containing 0.05 % sodium azide

Preservative: Other preservative, Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Storage: 4 °C

Storage Comment:

Stable at room temperature or 37°C for 7 days.

Protect from light,

Store at 2 to 8°C. Protect fluorescent conjugates from light

Expiry Date: 24 months

Target Details for Calnexin

Target: Calnexin (CANX)

Alternative Name: Calnexin

Background:

Synonyms: Calnexin, CANX, CNX, IP90, Major histocompatibility complex class I antigen-binding protein p88, P90

Keywords: exosome, vesicle, western, exobrite

Gene Symbol: CANX

Tissue Expression: All cells

Molecular Weight: 67 kDa (predicted), 80-90 kDa (observed)

Gene ID: 821

UniProt: P27824

Pathways: MAPK Signaling, Thyroid Hormone Synthesis