CD22 antibody (AA 52-178) (CF®568)

Catalog No. ABIN7651006

This Mouse Monoclonal antibody specifically detects CD22 in FACS, WB and IF. It exhibits reactivity toward Human.

Quick Overview for CD22 antibody (AA 52-178) (CF®568) (ABIN7651006)

Target: CD22 (CD22 Molecule (CD22))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD22 antibody is conjugated to CF®568

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunofluorescence (IF)

Clone: BLCAM-1796

Product Details anti-CD22 Antibody (CF®568)

Binding Specificity: AA 52-178

Purpose: CD22 / BL-CAM (B-Cell Marker) (BLCAM/1796), CF568 conjugate

Characteristics: This antibody recognizes a protein of 130-140 kDa, identified as CD22 (also known as BL-CAM). CD22 expression is restricted to normal and neoplastic B cells and is absent from other haemopoietic cell types. In B-cell ontogeny, CD22 is first expressed in the cytoplasm of pro-B and pre-B cells, and on the surface as B cells mature to become IgD . It is not expressed by plasma cells, CD22 is found highly expressed in follicular mantle and marginal zone B-cells, and while germinal center B-cells are relatively weak. CD22 is a member of the immunoglobulin superfamily and serves as an adhesion receptor for sialic acid-bearing ligands expressed on erythrocytes and all leukocyte classes. It also associates with tyrosine kinases and play a role in signal transduction and B-cell activation.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant fragment of human CD22 protein (around aa 52-178)

Isotype: IgG2b, kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Positive Control: Raji or Ramos cells (IF/FACS). Raji cell lysate (WB). Human tonsil, lymph node or spleen (IHC).

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % BSA, 0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Storage: 4 °C

Storage Comment:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Expiry Date: 24 months

Target Details for CD22

Target: CD22 (CD22 Molecule (CD22))

Alternative Name: BL-CAM

Background:

Synonyms: B-lymphocyte cell adhesion molecule (BL-CAM), B-cell receptor CD22, CD22, Lectin 2, Lyb8, Sialic acid-binding Ig-like lectin 2 (Siglec-2), SIGLEC2, T-cell surface antigen Leu-14

Gene Symbol: CD22

Tissue Expression: B-cells|Lymphocytes

Molecular Weight: 130-140 kDa

Gene ID: 933

UniProt: P20273