CD36 antibody (CF®640R)

Catalog No. ABIN7651304

The Mouse Monoclonal anti-CD36 antibody has been validated for WB, IP and Func. It is suitable to detect CD36 in samples from Human.

Quick Overview for CD36 antibody (CF®640R) (ABIN7651304)

Target: CD36

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD36 antibody is conjugated to CF®640R

Application: Western Blotting (WB), Immunoprecipitation (IP), Functional Studies (Func)

Clone: 1A7

Product Details anti-CD36 Antibody (CF®640R)

Purpose: CD36 (Platelet & Microvessel Marker)(1A7), CF640R conjugate

Characteristics: This antibody recognizes a protein of 80-90 kDa, identified as CD36. It is expressed on platelets, monocytes and macrophages, microvascular endothelial cells, erythrocyte precursors, mammary epithelial cells, and some macrophage derived dendritic cells. CD36 acts as a receptor for thrombospondin (TSP), collagen types I, IV and V, P. falciparum malaria-infected erythrocytes, and sickle erythrocytes. It also functions as a scavenger receptor, mediating macrophage uptake of oxidized low-density lipoprotein (LDL) and recognition of apoptotic polymorphonuclear leukocytes (PMN). CD36 plays a role in platelet aggregation, macrophage foam cell development, inflammation, and the tissue ischemia observed in sickle cell disease and cerebral malaria. Note that 1-4 % of Japanese and East Asia population lack CD36.

Immunogen: Human CD36 from platelets

Isotype: IgG2b, kappa

Application Details

Application Notes: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Flow cytometry: 0.5-1 μg/million cells. Functional Studies Order Ab without Azide. Immunofluorescence: 0.5-1 μg/mL. Optimal dilution for a specific application should be determined by user

Comment:

Positive Control: HEL or U937 cells. Platelets, monocytes, macrophages, microvascular endothelial cells in a tonsil.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % BSA, 0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Storage: 4 °C

Storage Comment:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Expiry Date: 24 months

Target Details for CD36

Target: CD36

Alternative Name: CD36

Background:

Synonyms: Adipocyte membrane protein, CHDS7, Collagen receptor, platelet, fatty acid translocase (FAT), Fatty acid transport protein, glycoprotein iiib (GP IIIb), PAS IV, PAS-4, pas4 protein, platelet collagen receptor, Platelet glycoprotein 4, scarb3, Scavenger receptor class B member 3, thrombospondin receptor

Gene Symbol: CD36

Tissue Expression: Platelets|Erythrocyte precursors|Mammary gland|Microvascular endothelial cells|Monocytes/macrophages

Molecular Weight: 80-90 kDa

Gene ID: 948

UniProt: P16671

Pathways: TLR Signaling, Peptide Hormone Metabolism, Response to Growth Hormone Stimulus, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Regulation of Lipid Metabolism by PPARalpha, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Hepatitis C, Toll-Like Receptors Cascades, Lipid Metabolism, S100 Proteins