CD46 antibody (CF®740)

Catalog No. ABIN7651624

This Mouse Monoclonal antibody specifically detects CD46 in FACS and Func. It exhibits reactivity toward Human.

Quick Overview for CD46 antibody (CF®740) (ABIN7651624)

Target: CD46

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD46 antibody is conjugated to CF®740

Application: Flow Cytometry (FACS), Functional Studies (Func)

Clone: 197-2B1

Product Details anti-CD46 Antibody (CF®740)

Purpose: CD46(197-2B1), CF740 conjugate

Characteristics: Recognizes a protein of 52 kDa-58 kDa, identified as CD46 (also known as membrane cofactor protein, MCP). CD46 exists as many isoforms in a variety of tissues. It is strongly expressed on salivary gland ducts and kidney ducts, moderately on lymphocytes and endothelium, and weakly on interstitial tissues and muscle cells, but not on erythrocytes. CD46 functions as a C3b/C4b-binding glycoprotein that inhibits complement activation on host cells. It also serves as a measles virus receptor, an adherence factor for group A Streptococcus pyogenes, and a cellular pilus receptor for pathogenic Neisseria. This MAb can be applied to test complement activation in pseudo-allergic reactions to acetylsalicylic acid and to test for measles virus infection of cells.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Stimulated human leukocytes

Isotype: IgG2a, kappa

Application Details

Application Notes: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Immunofluorescence: 0.5-1 μg/mL. Flow Cytometry 0.5-1 μg/million cells/0.1 mL. Optimal dilution for a specific application should be determined by user

Comment:

Positive Control: K562, U937, HL60, HeLa and Jurkat cells, and human lymphocytes. Lymph node and tonsil.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % rBSA, 0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Storage: 4 °C

Storage Comment:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Expiry Date: 24 months

Target Details for CD46

Target: CD46

Alternative Name: CD46

Background:

Synonyms: AHUS2, Complement membrane cofactor protein, MCP, Measles virus receptor, Membrane cofactor protein, MIC10, TLX, Trophoblast leucocyte common antigen, Trophoblast lymphocyte cross reactive antigen

Gene Symbol: CD46

Molecular Weight: 52-58 kDa

Gene ID: 4179

UniProt: P15529

Pathways: Regulation of Actin Filament Polymerization