CD80 antibody (AA 35-142) (Biotin)

Catalog No. ABIN7652062

This Mouse Monoclonal antibody specifically detects CD80 in . It exhibits reactivity toward Human.

Quick Overview for CD80 antibody (AA 35-142) (Biotin) (ABIN7652062)

Target: CD80

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD80 antibody is conjugated to Biotin

Application: Please inquire

Clone: C80-2725

Product Details anti-CD80 Antibody (Biotin)

Binding Specificity: AA 35-142

Purpose: CD80 (B7-1)(C80/2725), Biotin conjugate

Characteristics: T cell proliferation and lymphokine production are triggered by occupation of the TCR by antigen, followed by a costimulatory signal that is delivered by a ligand expressed on antigen presenting cells. The B7-related cell surface proteins CD80 (B7-1) and CD86 (B7-2) are expressed on antigen presenting cells bind the homologous T cell receptors CTLA-4 (cytotoxic T lymphocyte-associated protein-4) and CD28 and trigger costimulatory signals for optimal T cell activation. CTLA-4 shares 31 % overall amino acid identity with CD28 and it has been proposed that CD28 and CTLA-4 are functionally redundant. SLAM is a novel receptor on T cells that, when engaged, potentiates T cell expansion in a CD28-independent manner. B7, also designated BB1, is another ligand or counter receptor for CD28 and CTLA-4 that is expressed on the antigen-presenting cell. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant fragment of human CD80 protein (around aa 35-142)

Isotype: IgG2b, kappa

Application Details

Application Notes: For coating for ELISA, order Ab without BSA. Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Optimal dilution and staining procedure for a specific application should be determined by user. Recommended starting concentrations for titration are 1-2 μg/mL for most applications, or 1 μg/million cells/100 μLfor flow cytometry

Comment:

Positive Control: Raji or Ramos Cells. Tonsil, Spleen or Thymus

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % BSA, 0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment:

Stable at room temperature or 37°C for 7 days.

Store at 2 to 8°C. Protect fluorescent conjugates from light

Expiry Date: 24 months

Target Details for CD80

Target: CD80

Alternative Name: CD80

Background:

Synonyms: Activation B7-1 antigen, B lymphocyte activation antigen B7, B7, B7-1, BB1, CD28 antigen ligand 1, CD28LG, CD28LG1, CD80, Costimulatory factor CD80, CTLA-4 counter-receptor B7.1, LAB7, T-lymphocyte activation antigen CD80

Gene Symbol: CD80

Tissue Expression: Antigen presenting cells

Molecular Weight: 60 kDa

Gene ID: 941

UniProt: P33681

Pathways: TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Positive Regulation of Immune Effector Process, Cancer Immune Checkpoints