GM-CSF antibody (CF®740)

Catalog No. ABIN7652488

This Rat Monoclonal antibody specifically detects GM-CSF in ELISA, FACS and IF. It exhibits reactivity toward Human, Cynomolgus and Rhesus Monkey.

Quick Overview for GM-CSF antibody (CF®740) (ABIN7652488)

Target: GM-CSF (CSF2) (Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2))

Reactivity: Human, Cynomolgus, Rhesus Monkey

Host: Rat

Clonality: Monoclonal

Conjugate: This GM-CSF antibody is conjugated to CF®740

Application: ELISA, Flow Cytometry (FACS), Immunofluorescence (IF)

Clone: BVD2-21C11

Product Details anti-GM-CSF Antibody (CF®740)

Purpose: GM-CSF(BVD2-21C11), CF740 conjugate

Characteristics: Granulocyte/macrophage colony-stimulating factor (GM-CSF) is a hematopoietic factor that is produced by activated T-cells, B-cells, mast cells, macrophages, fibroblasts, and endothelial cells. In addition to supporting colony formation of granulocyte/macrophage progenitors, GM-CSF is a growth factor for erythroid, megakaryocyte, and eosinophil progenitors.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant human GM-CSF protein

Isotype: IgG2a, kappa

Application Details

Application Notes: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Immunoprecipitation: 0.5-1 μg/500 μg protein lysate. Immunofluorescence: 0.5-1 μg/mL. Immunohistology (frozen) 0.5-1 μg/mL. Flow Cytometry 0.5-1 μg/million cells/0.1 mL. Western blotting 0.5-1 μg/mL. Neutralization Studies order Ab without azide. Optimal dilution for a specific application should be determined by user

Comment:

Positive Control: Lymph node and tonsil

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % rBSA, 0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Storage: 4 °C

Storage Comment:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Expiry Date: 24 months

Target Details for GM-CSF

Target: GM-CSF (CSF2) (Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2))

Alternative Name: GM-CSF

Background:

Synonyms: Burst Promoting Activity, Eosinophil Colony Stimulating Factor, Molgramostin, Pluripoietin Alpha, Sargramostim

Gene Symbol: CSF2

Molecular Weight: 22 kDa

Gene ID: 1437

UniProt: P04141

Pathways: JAK-STAT Signaling, Cellular Response to Molecule of Bacterial Origin