Cyclin B2 antibody (CF®647)

Catalog No. ABIN7652745

This anti-Cyclin B2 antibody is a Mouse Monoclonal antibody detecting Cyclin B2 in IF. Suitable for Human, Mouse, Rat and Xenopus laevis.

Quick Overview for Cyclin B2 antibody (CF®647) (ABIN7652745)

Target: Cyclin B2 (CCNB2)

Reactivity: Human, Mouse, Rat, Xenopus laevis

Host: Mouse

Clonality: Monoclonal

Conjugate: This Cyclin B2 antibody is conjugated to CF®647

Application: Immunofluorescence (IF)

Clone: X29-2

Product Details anti-Cyclin B2 Antibody (CF®647)

Purpose: Cyclin B2(X29.2), CF647 conjugate

Characteristics: In eukaryotic cells, mitosis is initiated following the activation of a protein kinase known variously as maturation-promoting factor, M phase specific histone kinase or M-phase kinase. This protein kinase is composed of a catalytic subunit (Cdc2), a regulatory subunit (cyclin B) and a low molecular weight subunit (p13-Suc1). The Cdc/cyclin enzyme is subject to multiple levels of control, of which the regulation of the catalytic subunit by tyrosine phosphorylation is the best understood. Tyrosine phosphorylation inhibits the Cdc2/ cyclin B enzyme, and tyrosine dephosphorylation, occurring at the onset of mitosis, directly activates the pre-MPF complex. Evidence has established that B type cyclins not only act on M phase regulatory subunits of the Cdc2 protein kinase, but also activate the Cdc25A and Cdc25B endogenous tyrosine phosphatase, of which Cdc2 is the physiological substrate. The two B type cyclins, cyclin B1 and cyclin B2, have been shown to have distinct tissue distributions. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant full-length Xenopus laevis Cyclin B2 protein

Isotype: IgG1, kappa

Application Details

Application Notes: ELISA: For coating, order antibody without BSA, Optimal dilution for a specific application should be determined.. Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody

Comment:

Positive Control: HeLa cells. Human cervical tissue (IHC).

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % BSA, 0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Storage: 4 °C

Storage Comment:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Expiry Date: 24 months

Target Details for Cyclin B2

Target: Cyclin B2 (CCNB2)

Alternative Name: Cyclin B2

Background:

Synonyms: ccnb2, CycB2, Cyclin B2, G2 mitotic specific cyclin B2, HsT17299, MGC108931, MGC140694

Gene Symbol: CCNB2

Molecular Weight: 51 kDa

Gene ID: 9133

UniProt: O95067

Pathways: Cell Division Cycle, M Phase