CDK2 antibody (Biotin)

Catalog No. ABIN7652824

This Mouse Monoclonal antibody specifically detects CDK2 in WB. It exhibits reactivity toward Human, Mouse and Xenopus laevis.

Quick Overview for CDK2 antibody (Biotin) (ABIN7652824)

Target: CDK2 (Cyclin-Dependent Kinase 2 (CDK2))

Reactivity: Human, Mouse, Xenopus laevis

Host: Mouse

Clonality: Monoclonal

Conjugate: This CDK2 antibody is conjugated to Biotin

Application: Western Blotting (WB)

Clone: AN4-3

Product Details anti-CDK2 Antibody (Biotin)

Purpose: Cdk2 / p34cdc2 Serine-Threonine Kinase(AN4.3), Biotin conjugate

Characteristics: In vertebrates, as in yeast, multiple cyclins have been identified, including a total of eight such regulatory proteins in mammals. In contrast to the situation in yeast, the Cdc2 p34 kinase is not the only catalytic subunit identified in vertebrates that can interact with cyclins. While Cdc2 p34 is essential for the G2 to M transition in vertebrate cells, a second Cdc2-related kinase has also been implicated in cell cycle control. This protein, designated cyclindependent kinase 2 (Cdk2), also binds to cyclins and its kinase activity is temporally regulated during the cell cycle. Several additional Cdc2-related cyclin dependent kinases have been identified. These include Cdk3, Cdk4, Cdk5, PCTAIRE-1, PCTAIRE-2, PCTAIRE-3, Cdk6 Cdk7, Cdk8 and KKIALRE. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant full-length human Cdk2 protein

Isotype: IgG2a, kappa

Application Details

Application Notes: Inhibits activation of p34cdc2 kinase by cyclins, Immunoprecipitation, Kinase Assay, Western blot, Flow cytometry: 1-2 μg/mL, Optimal dilution for a specific application should be determined.

Comment:

Positive Control: HeLa cells. HeLa whole cell lysate.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % BSA, 0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment:

Stable at room temperature or 37°C for 7 days.

Store at 2 to 8°C. Protect fluorescent conjugates from light

Expiry Date: 24 months

Target Details for CDK2

Target: CDK2 (Cyclin-Dependent Kinase 2 (CDK2))

Alternative Name: Cdk2

Background:

Synonyms: Cdc2 related protein kinase, CDC28, CDC2A, CDK1, CDK2, CDKN2, Cell devision kinase 2, Cell division protein kinase 2, cyclin dependent kinase 2 alpha, kinase Cdc2, MPF, p33 protein kinase

Gene Symbol: CDK2

Molecular Weight: 34 kDa

Gene ID: 1017

UniProt: P24941

Pathways: PI3K-Akt Signaling, Cell Division Cycle, Mitotic G1-G1/S Phases, DNA Replication, M Phase, Synthesis of DNA