DNMT3A antibody (CF®594)

Catalog No. ABIN7653194

The Mouse Monoclonal anti-DNMT3A antibody has been validated for IHC and IHC (fp). It is suitable to detect DNMT3A in samples from Human.

Quick Overview for DNMT3A antibody (CF®594) (ABIN7653194)

Target: DNMT3A (DNA (Cytosine-5-)-Methyltransferase 3 alpha (DNMT3A))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This DNMT3A antibody is conjugated to CF®594

Application: Immunohistochemistry (IHC), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Clone: PCRP-DNMT3A-1E2

Product Details anti-DNMT3A Antibody (CF®594)

Purpose: DNMT3A / DNA Methyltransferase 3 Alpha (PCRP-DNMT3A-1E2), CF594 conjugate

Characteristics: The specificity of this monoclonal antibody to its intended target was validated by HuProt™,Array, containing more than 19,000, full-length human proteins. DNMT3A is required for genome-wide de novo methylation and is essential for the establishment of DNA methylation patterns during development. DNA methylation is coordinated with methylation of histones. It modifies DNA in a non-processive manner and also methylates non-CpG sites. May preferentially methylate DNA linker between 2 nucleosomal cores and is inhibited by histone H1. Plays a role in paternal and maternal imprinting. Required for methylation of most imprinted loci in germ cells. Acts as a transcriptional corepressor for ZBTB18. Recruited to trimethylated 'Lys-36' of histone H3 (H3K36me3) sites. Can actively repress transcription through the recruitment of HDAC activity. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant full-length human DNMT3A protein

Isotype: IgG1

Application Details

Application Notes: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Immunohistology (formalin): 1-2 μg/mL for 30 minutes at RT. Immunoprecipitation 2-4 μg/mg protein. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris with1 mM EDTA pH 9.0 for 10-20 minutes followed by cooling at RT for 20 minutes. Western blotting 0.5-1.0 μg/mL. Optimal dilution for a specific application should be determined by user

Comment:

Positive Control: HeLa cells orPlacenta

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % BSA, 0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Storage: 4 °C

Storage Comment:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Expiry Date: 24 months

Target Details for DNMT3A

Target: DNMT3A (DNA (Cytosine-5-)-Methyltransferase 3 alpha (DNMT3A))

Alternative Name: DNA Methyltransferase 3 Alpha

Background:

Synonyms: DNA methyltransferase 3 alpha, DNA methyltransferase HsaIIIA, DNAMTaseHsaIIIA, DNMT, DNMT3A2, M.HsaIIIA, MCMT, TBRS

Gene Symbol: DNMT3A

Molecular Weight: 102 kDa

Gene ID: 1788

UniProt: Q16655