Recombinant EGFRviii antibody (CF®405L)

Catalog No. ABIN7653355

This Rabbit Monoclonal antibody specifically detects EGFRviii in IHC and IHC (fp). It exhibits reactivity toward Human.

Quick Overview for Recombinant EGFRviii antibody (CF®405L) (ABIN7653355)

Target: EGFRviii (EGFRv3) (EGFR vIII (EGFRv3))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This EGFRviii antibody is conjugated to CF®405L

Application: Immunohistochemistry (IHC), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Clone: GFR-2600R

Product Details anti-EGFRviii Antibody (CF®405L)

Purpose: EGFRvIII (Epidermal Growth Factor Receptor, Variant III) (GFR/2600R), CF405L conjugate

Characteristics: EGFR is type I receptor tyrosine kinase with sequence homology to erbB-1, -2, -3 -4 or HER-1, -2, -3 -4. It binds to Epidermal Growth Factor (EGF), Transforming Growth Factor-a (TGF-a), Heparin-binding EGF (HB-EGF), amphiregulin, betacellulin and epiregulin. EGFR is overexpressed in tumors of breast, brain, bladder, lung, gastric, head & neck, esophagus, cervix, vulva, ovary, and endometrium. It is predominantly present in squamous cell carcinomas.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: KLH conjugate of EGFRvIII-specific, 14-amino acid peptide from the predicted amino acid sequence at the fusion junction (LEEKKGNYVVTDHC)

Isotype: IgG

Application Details

Application Notes: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Immunohistology (formalin): 1-2 μg/mL for 30 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined by user

Comment:

Positive Control: A431 cells. Placenta, Breast, Colon or Bladder Carcinoma

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % BSA, 0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Storage: 4 °C

Storage Comment:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Expiry Date: 24 months

Target Details for EGFRviii

Target: EGFRviii (EGFRv3) (EGFR vIII (EGFRv3))

Alternative Name: EGFRvIII

Background:

Synonyms: Erbb1 variant III, ERBB1 variant III, HER1 variant III, Proto-oncogene c-ErbB-1 variant III, Receptor Tyrosine Protein Kinase variant III, ErbB1 variant III

Gene Symbol: EGFR

Molecular Weight: ~145 kDa (vIII variant)

Gene ID: 1956

UniProt: P00533