Recombinant EGFRviii antibody

Catalog No. ABIN7862726

This Rabbit Monoclonal antibody specifically detects EGFRviii in IHC. It exhibits reactivity toward Human.

Quick Overview for Recombinant EGFRviii antibody (ABIN7862726)

Target: EGFRviii (EGFRv3) (EGFR vIII (EGFRv3))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This EGFRviii antibody is un-conjugated

Application: Immunohistochemistry (IHC)

Clone: GFR-2600R

Product Details anti-EGFRviii Antibody

Purpose: Recombinant EGFRvIII (Epidermal Growth Factor Receptor, Variant III) Antibody

Specificity: EGFR is type I receptor tyrosine kinase with sequence homology to erbB-1, -2, -3 -4 or HER-1, -2, -3 -4. It binds to Epidermal Growth Factor (EGF), Transforming Growth Factor-a (TGF-a), Heparin-binding EGF (HB-EGF), amphiregulin, betacellulin and epiregulin. EGFR is overexpressed in tumors of breast, brain, bladder, lung, gastric, head neck, esophagus, cervix, vulva, ovary, and endometrium. It is predominantly present in squamous cell carcinomas.

Purification: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G.

Immunogen: KLH conjugate of EGFRvIII-specific, 14-amino acid peptide from the predicted amino acid sequence at the fusion junction (LEEKKGNYVVTDHC)

Isotype: IgG, kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for EGFRviii

Target: EGFRviii (EGFRv3) (EGFR vIII (EGFRv3))

Alternative Name: EGFRvIII

Background:

Erbb1 variant III, ERBB1 variant III, HER1 variant III, Proto-oncogene c-ErbB-1 variant III, Receptor Tyrosine Protein Kinase variant III, ErbB1 variant III

A431 cells. Placenta, Breast, Colon or Bladder Carcinoma

Molecular Weight: ~145kDa (vIII variant)

Gene ID: 1956, 488293

UniProt: P00533