FLT3 antibody (CF®568)

Catalog No. ABIN7654124

The Mouse Monoclonal anti-FLT3 antibody has been validated for IHC and IHC (fp). It is suitable to detect FLT3 in samples from Human.

Quick Overview for FLT3 antibody (CF®568) (ABIN7654124)

Target: FLT3 (Fms-Related tyrosine Kinase 3 (FLT3))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This FLT3 antibody is conjugated to CF®568

Application: Immunohistochemistry (IHC), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Clone: FLT3-2458

Product Details anti-FLT3 Antibody (CF®568)

Purpose: Flt3 / CD135(FLT3/2458), CF568 conjugate

Characteristics: Stem cell tyrosine kinase (STK-1) has been cloned from a CD34 hematopoietic stem cell enriched library and identified as the human homolog of a previously identified gene of Mouse origin designated either Flk-2 or Flt-3. The STK-1 cDNA encodes a protein of 993 amino acids with 85 % identity to Flt-3/Flk-2. STK-1 is a member of the type III receptor tyrosine kinase family that includes Kit (steel factor receptor), Fms and PDGF. STK-1 expression in blood and marrow is restricted to CD34 cells, a population greatly enriched for hematopoietic stem/progenitor cells. STK-1 antiserum recognizes two polypeptides in these cells. The Mouse homolog of STK-1, designated Flt-3/Flk-2, is expressed at high levels in hematopoietic cells and also in neural, gonadal, hepatic and placental tissues. It has been suggested that STK-1 and its murine homolog Flt-3/Flk-2 may function as growth factor receptors on hematopoietic stem and/or progenitor cells. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant full-length human FLT3 protein

Isotype: IgG2b, kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Positive Control: Human lymph node or MDAMB468 xenograft.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % BSA, 0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Storage: 4 °C

Storage Comment:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Expiry Date: 24 months

Target Details for FLT3

Target: FLT3 (Fms-Related tyrosine Kinase 3 (FLT3))

Alternative Name: CD135

Background:

Synonyms: CD135, CD135 antigen, Fetal liver kinase 2, FL cytokine receptor, Flk2, FLT-3, Flt3, FLT3_HUMAN, FMS like tyrosine kinase 3, Fms related tyrosine kinase 3, Growth factor receptor tyrosine kinase type III, Ly-72, OTTHUMP0000004234, Receptor type tyrosine protein kinase FLT3, Stem cell tyrosine kinase 1, Stk 1, STK-1, Stk1, Tyrosine protein kinase receptor FLT3,

Gene Symbol: FLT3

Molecular Weight: 160 kDa

Gene ID: 2322

UniProt: P36888

Pathways: RTK Signaling