GNAQ antibody (CF®740)

Catalog No. ABIN7654738

This Mouse Monoclonal antibody specifically detects GNAQ in . It exhibits reactivity toward Human.

Quick Overview for GNAQ antibody (CF®740) (ABIN7654738)

Target: GNAQ (Guanine Nucleotide Binding Protein (G Protein), Q Polypeptide (GNAQ))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This GNAQ antibody is conjugated to CF®740

Application: Please inquire

Clone: GNAQ-2434

Product Details anti-GNAQ Antibody (CF®740)

Purpose: Guanine nucleotide-binding protein alpha-q / GNAQ / G-ALPHA-q(GNAQ/2434), CF740 conjugate

Characteristics: GNAQis 359 amino acids long and is identical in all but 1 amino acid residue to the mouse protein. Analysis of human genomic DNA revealed an intronless sequence with strong homology to human GNAQ cDNA. In comparison to GNAQ cDNA, this genomic DNA sequence included several small deletions and insertions that altered the reading frame, multiple single based changes, and a premature termination codon in the open reading frame, all hallmarks of a processed pseudogene. Probes derived from human GNAQ cDNA sequence mapped both chromosomes 2 and 9 in higher constringency genomic blot analyses of DNA from a panel of human/rodent hybrid cell lines. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant full-length human GNAQ protein

Isotype: IgG2b, kappa

Application Details

Application Notes: For coating for ELISA, order Ab without BSA. Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Optimal dilution and staining procedure for a specific application should be determined by user. Recommended starting concentrations for titration are 1-2 μg/mL for most applications, or 1 μg/million cells/100 μLfor flow cytometry

Comment:

Positive Control: HL-60, MOLT-4 and MCF-7 cells. Predominantly expressed in ovary, prostate, testis and colon.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % rBSA, 0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Storage: 4 °C

Storage Comment:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Expiry Date: 24 months

Target Details for GNAQ

Target: GNAQ (Guanine Nucleotide Binding Protein (G Protein), Q Polypeptide (GNAQ))

Alternative Name: G-Alpha-q

Background:

Synonyms: CMC1, G alpha Q, G protein alpha Q, GAQ, guanine nucleotide binding protein (G protein), q polypeptide, Guanine nucleotide-binding protein G(q) subunit alpha, SWS

Gene Symbol: GNAQ

Molecular Weight: 45 kDa

Gene ID: 2776

UniProt: P50148

Pathways: JAK-STAT Signaling, Thyroid Hormone Synthesis, Myometrial Relaxation and Contraction