ITGB1 antibody (CF®568)

Catalog No. ABIN7655419

This Mouse Monoclonal antibody specifically detects ITGB1 in FACS, IP, IF and Func. It exhibits reactivity toward Human.

Quick Overview for ITGB1 antibody (CF®568) (ABIN7655419)

Target: ITGB1 (Integrin beta 1 (ITGB1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This ITGB1 antibody is conjugated to CF®568

Application: Flow Cytometry (FACS), Immunoprecipitation (IP), Immunofluorescence (IF), Functional Studies (Func)

Clone: 12G10

Product Details anti-ITGB1 Antibody (CF®568)

Purpose: CD29 (Stem Cell Marker)(12G10), CF568 conjugate

Characteristics: Recognizes CD29. Binds to the cell membrane of T- and B- lymphocytes, monocytes, platelets, fibroblasts, endothelial cells, mast cells and smooth muscle cells. CD29 is an adhesion receptor and co-stimulatory molecule. This antigen was initially characterized as GPIIa on platelets and as the common b1 subunit of the very late antigen (VLA) protein family. CD29 forms a non-covalent heterodimeric complex with integrin alpha subunits. It also mediates adhesion to invasion and thrombospondin. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Purified human beta 1 integrin from HT1080 fibrosarcoma cell extract.

Isotype: IgG1, kappa

Application Details

Application Notes: Does not react with Mouse or Rat, ELISA: For coating use Ab at 1-5 μg/mL, order Ab without BSA, Optimal dilution for a specific application should be determined by user.. Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody

Comment:

Positive Control: HT1080 whole cell lysate. HeLa cell line.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % BSA, 0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Storage: 4 °C

Storage Comment:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Expiry Date: 24 months

Target Details for ITGB1

Target: ITGB1 (Integrin beta 1 (ITGB1))

Alternative Name: CD29

Background:

Synonyms: Fibrinogen Receptor beta subunit, FNRB, Glycoprotein IIa (GPIIA), Integrin beta-1 (ITGB1), integrin VLA-4 beta subunit, MDF2, MSK12, Very Late Activation Protein beta Polypeptide, VLA-4 subunit beta, VLA-BETA, VLAB

Gene Symbol: ITGB1

Tissue Expression: Platelets|Endothelial cells|Fibroblasts|Lymphocytes|Mast cells|Monocytes/macrophages|Smooth muscle

Molecular Weight: 110 kDa (non-reduced) & 130 kDa (reduced)

Gene ID: 3688

UniProt: P05556

Pathways: Cell-Cell Junction Organization, Regulation of G-Protein Coupled Receptor Protein Signaling, CXCR4-mediated Signaling Events, Signaling of Hepatocyte Growth Factor Receptor, Integrin Complex, SARS-CoV-2 Protein Interactome