JUP antibody (CF®740)

Catalog No. ABIN7655815

This Mouse Monoclonal antibody specifically detects JUP in WB, IF and FACS. It exhibits reactivity toward Human, Mouse, Rat, Chicken, Cow, Dog and Cat.

Quick Overview for JUP antibody (CF®740) (ABIN7655815)

Target: JUP (Junction Plakoglobin (JUP))

Reactivity: Human, Mouse, Rat, Chicken, Cow, Dog, Cat

Host: Mouse

Clonality: Monoclonal

Conjugate: This JUP antibody is conjugated to CF®740

Application: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS)

Clone: 15F11

Product Details anti-JUP Antibody (CF®740)

Purpose: Catenin, gamma (Cardiomyocyte Marker) (15F11), CF740 conjugate

Characteristics: Gamma catenin, also known as JUP or junction plakoglobin, is a member of the catenin family. The catenins (alpha, beta, gamma, and delta) are ubiquitously expressed, cytoplasmic proteins that associate with E-cadherin at cellular junctions. Catenin/cadherin complexes play an important role in mediating cellular adhesion, including adherens junctions.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: full length chicken Catenin gamma protein|Recombinant

Isotype: IgG1, kappa

Application Details

Application Notes: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Immunofluorescence: 1-2 μg/mL. Flow Cytometry 0.5-1 μg/million cells/0.1 mL. Western blotting 0.5-1 μg/mL. Optimal dilution for a specific application should be determined by user

Comment:

Positive Control: A431, T47D or MCF-7 cells. Skin

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % rBSA, 0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Storage: 4 °C

Storage Comment:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Expiry Date: 24 months

Target Details for JUP

Target: JUP (Junction Plakoglobin (JUP))

Alternative Name: Gamma-catenin

Background:

Synonyms: Gamma catenin, JUP, Junction plakoglobin, Catenin gamma

Gene Symbol: JUP

Tissue Expression: Epithelial cells

Molecular Weight: 80-87 kDa

Gene ID: 3728

UniProt: P14923

Pathways: Cell-Cell Junction Organization, Maintenance of Protein Location