JUP antibody (CF®740)

Catalog No. ABIN7655816

The Mouse Monoclonal anti-JUP antibody has been validated for IHC and IHC (fp). It is suitable to detect JUP in samples from Human.

Quick Overview for JUP antibody (CF®740) (ABIN7655816)

Target: JUP (Junction Plakoglobin (JUP))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This JUP antibody is conjugated to CF®740

Application: Immunohistochemistry (IHC), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Clone: CTNG-1483

Product Details anti-JUP Antibody (CF®740)

Purpose: Catenin, gamma (Cardiomyocyte Marker) (CTNG/1483), CF740 conjugate

Characteristics: Gamma catenin, also known as JUP or junction plakoglobin, is a member of the catenin family. The catenins (alpha, beta, gamma, and delta) are ubiquitously expressed, cytoplasmic proteins that associate with E-cadherin at cellular junctions. Catenin/cadherin complexes play an important role in mediating cellular adhesion, including adherens junctions.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant human full-length gamma catenin protein

Isotype: IgG1, kappa

Application Details

Application Notes: Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris, 1 mM EDTA pH 9.0 for 10-20 min followed by cooling at RT for 20 min. Immunohistology (formalin) 0.1-0.2 μg/mL. Flow Cytometry 0.5-1 μg/million cells/0.1 mL. Immunofluorescence 1-2 μg/mL. Western blotting 0.5-1 μg/mL. Optimal dilution for a specific application should be determined by user

Comment:

Positive Control: A431, T47D or MCF-7 cells. Skin

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % rBSA, 0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Storage: 4 °C

Storage Comment:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Expiry Date: 24 months

Target Details for JUP

Target: JUP (Junction Plakoglobin (JUP))

Alternative Name: Gamma-catenin

Background:

Synonyms: Gamma catenin, JUP, Junction plakoglobin, Catenin gamma

Gene Symbol: JUP

Tissue Expression: Epithelial cells

Molecular Weight: 80-87 kDa

Gene ID: 3728

UniProt: P14923

Pathways: Cell-Cell Junction Organization, Maintenance of Protein Location