MMP2 antibody (AA 444-575) (CF®740)

Catalog No. ABIN7656706

This Mouse Monoclonal antibody specifically detects MMP2 in WB. It exhibits reactivity toward Human.

Quick Overview for MMP2 antibody (AA 444-575) (CF®740) (ABIN7656706)

Target: MMP2 (Matrix Metalloproteinase 2 (MMP2))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This MMP2 antibody is conjugated to CF®740

Application: Western Blotting (WB)

Clone: MMP2-1501

Product Details anti-MMP2 Antibody (CF®740)

Binding Specificity: AA 444-575

Purpose: MMP2 / Collagenase Type IV A (Tumor Metastasis Marker) (MMP2/1501), CF740 conjugate

Characteristics: This antibody recognizes a protein of 72 kDa, which is identified as MMP2. The matrix metalloproteinases (MMP) are a family of peptidase enzymes responsible for the degradation of extracellular matrix components, including collagen, gelatin, Fibronectin, Laminin and proteoglycan. Transcription of MMP genes is differentially activated by phorbol ester, lipopolysaccharide (LPS) or staphylococcal enterotoxin B (SEB). MMP catalysis requires both calcium and zinc. MMP-2 (also designated type IV collagenase) cleaves collagen types IV,V, VII and X and gelatin type I. Activation of MMP-2 secretion requires the Ras signaling pathway. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant human MMP2 protein fragment (aa444-575)

Isotype: IgG1

Application Details

Application Notes: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Immunofluorescence: 0.5-1 μg/mL. Flow Cytometry 0.5-1 μg/million cells/0.1 mL. Western blotting 0.5-1 μg/mL. Optimal dilution for a specific application should be determined by user

Comment:

Positive Control: U-138 MG or U-87 MG cells. Placenta

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % rBSA, 0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Storage: 4 °C

Storage Comment:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Expiry Date: 24 months

Target Details for MMP2

Target: MMP2 (Matrix Metalloproteinase 2 (MMP2))

Alternative Name: Collagenase-Type-IV-A

Background:

Synonyms: Type IV collagenase|CLG4A|Collagenase Type 4 alpha|Collagenase type IV A|Gelatinase A|Gelatinase alpha|Gelatinase neutrophil|Matrix metalloproteinase-2|MMP2|MONA|Neutrophil gelatinase|PEX|TBE-1

Gene Symbol: MMP2

Molecular Weight: 72 kDa (Pro), 63 kDa (cleaved)

Gene ID: 4313

UniProt: P08253

Pathways: Activation of Innate immune Response