MHC Class II HLA-DP/DQ/DR antibody (CF®568)

Catalog No. ABIN7657067

The Mouse Monoclonal anti-MHC Class II HLA-DP/DQ/DR antibody is suitable to detect MHC Class II HLA-DP/DQ/DR in samples from Human. It has been validated for FACS, IF, WB, IHC and IHC (fp).

Quick Overview for MHC Class II HLA-DP/DQ/DR antibody (CF®568) (ABIN7657067)

Target: MHC Class II HLA-DP/DQ/DR (HLA-DP/DQ/DR)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This MHC Class II HLA-DP/DQ/DR antibody is conjugated to CF®568

Application: Flow Cytometry (FACS), Immunofluorescence (IF), Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Clone: CR3-43

Product Details anti-MHC Class II HLA-DP/DQ/DR Antibody (CF®568)

Purpose: HLA-DP/-DQ/-DR (MHC II)(CR3/43), CF568 conjugate

Characteristics: Reacts with a common epitope of human major histocompatibility (MHC) class II antigens, HLA-DP, -DQ and -DR. Human MHC class II antigens are transmembrane glycoproteins composed of an chain (36 kDa) and a chain (27 kDa). They are expressed primarily on antigen presenting cells such as B lymphocytes, monocytes, macrophages, and thymic epithelial cells and are also present on activated T lymphocytes. Human MHC class II genes are located in the HLA-D region that encodes at least six and ten chain genes. Three loci, DR, DQ and DP, encode the major expressed products of the human class II region. The human MHC class II molecules bind intracellularly processed peptides and present them to T-helper cells. They, therefore, have a critical role in the initiation of the immune response. It has been shown that some autoimmune diseases are associated with certain class II alleles. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Cells from human tonsil

Isotype: IgG1, kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Positive Control: Ramos, Raji cells. Tonsil, Spleen or lymph node.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % BSA, 0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Storage: 4 °C

Storage Comment:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Expiry Date: 24 months

Target Details for MHC Class II HLA-DP/DQ/DR

Target: MHC Class II HLA-DP/DQ/DR (HLA-DP/DQ/DR)

Alternative Name: HLA-DP/-DQ/-DR

Background:

Synonyms: HLA-DPB1, HLA-DQA1, HLA-DQB1, HLA-DRA, HLA-DRB1, HLA-DRB3, HLA-DRB4, HLA-DRB5,

Gene Symbol: HLA-DP, HLA-DQ, HLA-DR

Molecular Weight: 36 kDa (? chain) and 27 kDa (? chain)

Gene ID: 3115, 3117, 3122

UniProt: P04440, P01908, P01909, P01920, P01903

Pathways: Human Leukocyte Antigen (HLA) in Adaptive Immune Response