MCM6 antibody (AA 228-368)

Catalog No. ABIN7657134

This anti-MCM6 antibody is a Mouse Monoclonal antibody detecting MCM6 in IHC and IHC (fp). Suitable for Human.

Quick Overview for MCM6 antibody (AA 228-368) (ABIN7657134)

Target: MCM6 (Minichromosome Maintenance Complex Component 6 (MCM6))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This MCM6 antibody is un-conjugated

Application: Immunohistochemistry (IHC), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Clone: MCM6-3000

Product Details anti-MCM6 Antibody

Binding Specificity: AA 228-368

Purpose: MCM6 (Proliferation Marker)(MCM6/3000)

Characteristics: The mini-chromosome maintenance (MCM) family of proteins, including MCM2, MCM3, MCM6 (Cdc21), MCM5 (Cdc46), MCM6 (Mis5) and MCM7 (Cdc47), are regulators of DNA replication that act to ensure replication occurs only once in the cell cycle. Expression of MCM proteins increases during cell growth, peaking at G1/S phase. The MCM proteins each contain an ATP-binding motif, which is predicted to mediate ATP-dependent opening of double-stranded DNA. MCM proteins are regulated by E2F transcription factors, which induce MCM expression, and by protein kinases, which interact with MCM proteins to maintain the post-replicative state of the cell. MCM2/MCM6 complexes function as substrates for Cdc2/cyclin B in vitro. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant fragment (around aa 228-368) of human MCM6 protein

Isotype: IgG2b, kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Positive Control: Human skin or squamous cell carcinoma (IHC).

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: PBS, 0.05 % BSA, 0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment:

Stable at room temperature or 37°C for 7 days.

Store at 2 to 8°C. Protect fluorescent conjugates from light

Expiry Date: 24 months

Target Details for MCM6

Target: MCM6 (Minichromosome Maintenance Complex Component 6 (MCM6))

Alternative Name: MCM6

Background:

Synonyms: DNA replication licensing factor MCM6, MCM6 minichromosome maintenance deficient 6, MIS5 homolog, p105MCM

Gene Symbol: MCM6

Molecular Weight: 105 kDa

Gene ID: 4175

UniProt: Q14566

Pathways: DNA Damage Repair, Mitotic G1-G1/S Phases, DNA Replication, Synthesis of DNA