MCM6 antibody (AA 228-368)

Catalog No. ABIN6940016

The Mouse Monoclonal anti-MCM6 antibody is suitable to detect MCM6 in samples from Human, Rat, Guinea Pig and Hamster. It has been validated for IHC and WB.

Quick Overview for MCM6 antibody (AA 228-368) (ABIN6940016)

Target: MCM6 (Minichromosome Maintenance Complex Component 6 (MCM6))

Reactivity: Human, Rat, Guinea Pig, Hamster

Host: Mouse

Clonality: Monoclonal

Conjugate: This MCM6 antibody is un-conjugated

Application: Immunohistochemistry (IHC), Western Blotting (WB)

Clone: MCM6-2999

Product Details anti-MCM6 Antibody

Binding Specificity: AA 228-368

Purpose: MCM6 (Proliferation Marker) Antibody

Specificity: The mini-chromosome maintenance (MCM) family of proteins, including MCM2, MCM3, MCM6 (Cdc21), MCM5 (Cdc46), MCM6 (Mis5) and MCM7 (Cdc47), are regulators of DNA replication that act to ensure replication occurs only once in the cell cycle. Expression of MCM proteins increases during cell growth, peaking at G1/S phase. The MCM proteins each contain an ATP-binding motif, which is predicted to mediate ATP-dependent opening of double-stranded DNA. MCM proteins are regulated by E2F transcription factors, which induce MCM expression, and by protein kinases, which interact with MCM proteins to maintain the post-replicative state of the cell. MCM2/MCM6 complexes function as substrates for Cdc2/cyclin B in vitro.

Purification: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Recombinant fragment (around aa 228-368) of human MCM6 protein (exact sequence is proprietary)

Isotype: IgG2b, kappa

Application Details

Application Notes:

Positive Control: Human skin or squamous cell carcinoma (IHC).

Known Application: Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for MCM6

Target: MCM6 (Minichromosome Maintenance Complex Component 6 (MCM6))

Alternative Name: MCM6

Background:

DNA replication licensing factor MCM6, MCM6 minichromosome maintenance deficient 6, MIS5 homolog, p105MCM

A-431, Human skin or squamous cell carcinoma (IHC). Human Kidney, Mouse Kidney, Rat Kidney, Hamster Kidney or Guinea pig Kidney

Molecular Weight: 105kDa

Gene ID: 4175, 444118

UniProt: Q14566

Pathways: DNA Damage Repair, Mitotic G1-G1/S Phases, DNA Replication, Synthesis of DNA