Recombinant MCM7 antibody (AA 195-319) (CF®405S)

Catalog No. ABIN7657139

The Mouse Monoclonal anti-MCM7 antibody has been validated for WB, IHC and IHC (fp). It is suitable to detect MCM7 in samples from Human.

Quick Overview for Recombinant MCM7 antibody (AA 195-319) (CF®405S) (ABIN7657139)

Target: MCM7 (Minichromosome Maintenance Complex Component 7 (MCM7))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This MCM7 antibody is conjugated to CF®405S

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Clone: RMCM7-1468

Product Details anti-MCM7 Antibody (CF®405S)

Binding Specificity: AA 195-319

Purpose: MCM7 (Proliferation Marker)(rMCM7/1468), CF405S conjugate

Characteristics: The specificity of this monoclonal antibody to its intended target was validated by HuProt™, Array, containing more than 19, 000, full-length human proteins. MCM7 is one of the highly conserved mini-chromosome maintenance proteins (MCM) that is essential for the initiation of eukaryotic genome replication. The hexameric protein complex formed by the MCM proteins is a key component of the pre-replication complex and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. The MCM complex consisting of this protein and MCM2, 4 and 6 proteins possesses DNA helicase activity, and may act as a DNA unwinding enzyme. Cyclin D1-dependent kinase, CDK4, is found to associate with this protein, and may regulate the binding of this protein with the tumor suppressor protein RB1/RB. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant human MCM7 protein fragment (aa195-319)

Isotype: IgG1, kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Positive Control: MCF-7, HEK-293, A549, SKBr3, HeP2, Raji or Ramos cells. Tonsil (IHC).

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % BSA, 0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Storage: 4 °C

Storage Comment:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Expiry Date: 24 months

Target Details for MCM7

Target: MCM7 (Minichromosome Maintenance Complex Component 7 (MCM7))

Alternative Name: MCM7

Background:

Synonyms: CDC47, DNA replication licensing factor MCM7, MCM7 mini chromosome maintenance deficient 7, Mini chromosome Maintenance 7, Mini chromosome maintenance protein 7, P1.1-MCM3, P1CDC47, P85MCM, PNAS146

Gene Symbol: MCM7

Molecular Weight: 88 kDa

Gene ID: 4176

UniProt: P33993

Pathways: DNA Damage Repair, Mitotic G1-G1/S Phases, DNA Replication, Chromatin Binding, Synthesis of DNA