MSH2 antibody (AA 327-427) (CF®568)

Catalog No. ABIN7657174

This Mouse Monoclonal antibody specifically detects MSH2 in WB, IHC, IF, FACS and IHC (fp). It exhibits reactivity toward Human.

Quick Overview for MSH2 antibody (AA 327-427) (CF®568) (ABIN7657174)

Target: MSH2 (Mismatch Repair Protein 2 (MSH2))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This MSH2 antibody is conjugated to CF®568

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Clone: MSH2-2622

Product Details anti-MSH2 Antibody (CF®568)

Binding Specificity: AA 327-427

Purpose: MSH2 (DNA Mismatch Repair Marker)(MSH2/2622), CF568 conjugate

Characteristics: Mutations in DNA mismatch repair genes are associated with hereditary nonpolyposis colorectal cancer (HNPCC). Initially, inherited mutations in the MSH2 and MLH1 homologs of the bacterial DNA mismatch repair genes MutS and MutL were found at high frequency in HNPCC and were shown to be associated with microsatellite instability. The demonstration that 10 to 45 % of pancreatic, gastric, breast, ovarian and small cell lung cancers also display microsatellite instability has been interpreted to suggest that DNA mismatch repair is not restricted to HNPCC tumors but is a common feature in tumor initiation or progression. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant fragment (around aa 327-427) of human MSH2 protein

Isotype: IgG1, kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Positive Control: A549 or HepG2 cells. Colon, Thyroid, Testis or Lymph Node.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % BSA, 0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Storage: 4 °C

Storage Comment:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Expiry Date: 24 months

Target Details for MSH2

Target: MSH2 (Mismatch Repair Protein 2 (MSH2))

Alternative Name: MSH2

Background:

Synonyms: BAT26, COCA1, DNA mismatch repair protein Msh2, FCC1, hMSH2, HNPCC1, LCFS2, MSH2, MutS homolog 2, MutS homolog 2 colon cancer nonpolyposis type 1, MutS protein homolog 2

Gene Symbol: MSH2

Molecular Weight: 100 kDa

Gene ID: 4436

Pathways: DNA Damage Repair, Production of Molecular Mediator of Immune Response