NADPH Oxidase 4 antibody (CF®740)

Catalog No. ABIN7657692

This anti-NADPH Oxidase 4 antibody is a Mouse Monoclonal antibody detecting NADPH Oxidase 4 in . Suitable for Human.

Quick Overview for NADPH Oxidase 4 antibody (CF®740) (ABIN7657692)

Target: NADPH Oxidase 4 (NOX4)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This NADPH Oxidase 4 antibody is conjugated to CF®740

Application: Please inquire

Clone: NOX4-1245

Product Details anti-NADPH Oxidase 4 Antibody (CF®740)

Purpose: NOX4 / NADPH Oxidase 4 (NOX4/1245), CF740 conjugate

Characteristics: The superoxide-generating NADPH oxidase includes a membrane-bound flavocytochrome containing two subunits, gp91-phox and p22-phox, and the cytosolic proteins p47-phox and p67-phox. During activation of the NADPH oxidase, p47-phox and p67-phox migrate to the plasma membrane where they associate with the flavocytochrome, cytochrome b558, to form the active enzyme complex. The p22 and gp91-phox subunits also function as surface O2 sensors that initiate cellular signaling in response to hypoxic conditions. NOX4 is a renal gp91-phox homolog highly expressed at the site of erythropoietin production in the proximal convoluted tubule epithelial cells of the renal cortex. It is also expressed in fetal tissues, placenta, glioblastoma and vascular cells.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant human NOX4 protein

Isotype: IgG2b, kappa

Application Details

Application Notes: For coating for ELISA, order Ab without BSA. Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Optimal dilution and staining procedure for a specific application should be determined by user. Recommended starting concentrations for titration are 1-2 μg/mL for most applications, or 1 μg/million cells/100 μLfor flow cytometry

Comment:

Positive Control: PC-12, JAR cells. Kidney

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % rBSA, 0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Storage: 4 °C

Storage Comment:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Expiry Date: 24 months

Target Details for NADPH Oxidase 4

Target: NADPH Oxidase 4 (NOX4)

Alternative Name: NADPH-Oxidase-4

Background:

Synonyms: Kidney oxidase-1, Kidney superoxide-producing NADPH oxidase, KOX-1, NADPH oxidase 4, Nox4, Renal NAD(P)H-oxidase, RENOX

Gene Symbol: NOX4

Tissue Expression: Endothelial cells|Kidney

Molecular Weight: 70 kDa

Gene ID: 50507

UniProt: Q9NPH5

Pathways: Carbohydrate Homeostasis, Smooth Muscle Cell Migration