Recombinant NGFR antibody (AA 281-421) (CF®640R)

Catalog No. ABIN7657772

The Mouse Monoclonal anti-NGFR antibody has been validated for WB, IHC and IHC (fp). It is suitable to detect NGFR in samples from Human.

Quick Overview for Recombinant NGFR antibody (AA 281-421) (CF®640R) (ABIN7657772)

Target: NGFR (Nerve Growth Factor Receptor (NGFR))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This NGFR antibody is conjugated to CF®640R

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Clone: RNGFR-1965

Product Details anti-NGFR Antibody (CF®640R)

Binding Specificity: AA 281-421

Purpose: NGF-Receptor (p75) / CD271 (Soft Tissue Tumor Marker)(rNGFR/1965), CF640R conjugate

Characteristics: Recognizes a glycoprotein of 75 kDa, identified as low affinity Nerve Growth Factor (NGF) Receptor (p75NGFR) or Neurotrophin Receptor (p75NTR). NGFR is expressed in various neural crest cells and their tumors such as melanocytes, melanomas, neuroblastomas, pheochromocytomas and neurofibromas. Reportedly, anti-NGFR is a reliable marker for desmoplastic and neurotropic melanomas. NGFR is expressed in mature non-neural cells such as perivascular cells, dental pulp cells, lymphoidal follicular dendritic cells, basal epithelium of oral mucosa and hair follicles, prostate basal cells, and myoepithelial cells. Anti-NGFR stains the myoepithelial cells of breast ducts and intra-lobular fibroblasts of breast ducts. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant human p75 NGFR protein fragment (around aa 281-421)

Isotype: IgG1, kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % BSA, 0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Storage: 4 °C

Storage Comment:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Expiry Date: 24 months

Target Details for NGFR

Target: NGFR (Nerve Growth Factor Receptor (NGFR))

Alternative Name: CD271

Background:

Synonyms: CD271, Gp80-LNGFR, Low affinity nerve growth factor receptor, Low affinity neurotrophin receptor p75NTR, Nerve growth factor receptor (NGFR), p75 ICD, p75 Neurotrophin receptor, Tumor necrosis factor receptor superfamily member 16 (TNFRSF16)

Gene Symbol: NGFR

Tissue Expression: Neural crest cells

Molecular Weight: 75 kDa

Gene ID: 4804

UniProt: P08138

Pathways: NF-kappaB Signaling, Neurotrophin Signaling Pathway, Carbohydrate Homeostasis, Growth Factor Binding