Recombinant Neurofilament antibody (CF®740)

Catalog No. ABIN7657877

The Mouse Monoclonal anti-Neurofilament antibody is suitable to detect Neurofilament in samples from Human, Rat, Mouse, Chicken and Pig. It has been validated for FACS, IHC, IHC (fp) and WB.

Quick Overview for Recombinant Neurofilament antibody (CF®740) (ABIN7657877)

Target: Neurofilament

Antibody Type: Recombinant Antibody

Reactivity: Human, Rat, Mouse, Chicken, Pig

Host: Mouse

Clonality: Monoclonal

Conjugate: This Neurofilament antibody is conjugated to CF®740

Application: Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp)), Western Blotting (WB)

Clone: RNF421

Product Details anti-Neurofilament Antibody (CF®740)

Purpose: Neurofilament (NF-H) (Neuronal Marker) (rNF421), CF740 conjugate

Characteristics: This MAb reacts with a 200 kDa protein, identified as heavy sub-unit of neurofilaments (NF-H). Neurofilaments make up the main structural elements of axons and dendrites and are found in neurons, peripheral nerves, and sympathetic ganglion cells. Neurofilaments consist of three major subunits with molecular weights of 68 kDa (NF-L), 160 kDa (NF-M) and 200 kDa (NF-H). Anti-neurofilament stains a number of neural, neuroendocrine, and endocrine tumors. Neuromas, ganglioneuromas, gangliogliomas, ganglioneuroblastomas, and neuroblastomas stain positively for anti-neurofilament. Neurofilaments are also present in paragangliomas as well as adrenal and extra-adrenal pheochromocytomas. Carcinoids, neuroendocrine carcinomas of the skin, and oat cell carcinomas of the lung also express neurofilament.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant full-length human NEFH protein

Isotype: IgG1

Application Details

Application Notes: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Immunohistology (formalin): 0.5-1.0 μg/mL for 30 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes. Western blotting 1-2 μg/mL. Optimal dilution for a specific application should be determined by user

Comment:

Positive Control: Brain, Neuroblastoma

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % rBSA, 0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Storage: 4 °C

Storage Comment:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Expiry Date: 24 months

Target Details for Neurofilament

Target: Neurofilament

Background:

Synonyms: NEFH, Neurofilament H, Neurofilament Heavy Polypeptide 200 kDa, Neurofilament Triplet H Protein, NF-H, NF200

Gene Symbol: NEFH

Tissue Expression: Neuroendocrine cells|Neurons

Molecular Weight: 200 kDa

Gene ID: 4744

UniProt: P12036