Melanoma gp100 antibody (CF®740)

Catalog No. ABIN7658897

The Mouse Monoclonal anti-Melanoma gp100 antibody has been validated for IHC, IF, FACS, ELISA, IHC (fp) and IP. It is suitable to detect Melanoma gp100 in samples from Human and Horse.

Quick Overview for Melanoma gp100 antibody (CF®740) (ABIN7658897)

Target: Melanoma gp100 (PMEL) (Premelanosome Protein (PMEL))

Reactivity: Human, Horse

Host: Mouse

Clonality: Monoclonal

Conjugate: This Melanoma gp100 antibody is conjugated to CF®740

Application: Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), ELISA, Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp)), Immunoprecipitation (IP)

Clone: NKI-beteb

Product Details anti-Melanoma gp100 Antibody (CF®740)

Purpose: Pmel17 / gp100 / SILV(NKI-beteb), CF740 conjugate

Characteristics: By immunohistochemistry, this antibody specifically recognizes a protein in melanocytes and melanomas. It reacts with junctional and blue nevus cells and variably with fetal and neonatal melanocytes. Intradermal nevi, normal adult melanocytes, and non-melanocytic cells are negative. It does not stain tumor cells of epithelial, lymphoid, glial, or mesenchymal origin. This Mab labels formalin-fixed, paraffin-embedded melanomas and other tumors showing melanocytic differentiation.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Membranes from a human melanoma metastasis

Isotype: IgG2b, kappa

Application Details

Application Notes: Immunohistology formalin-fixed 1-2 μg/mL. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes. Flow Cytometry 0.5-1 μg/million cells/0.1 mL. Immunofluorescence 1-2 μg/mL. Does not react with rat, others not tested. Optimal dilution for a specific application should be determined by user

Comment:

Positive Control: SK-MEL-28 cells or Melanoma

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % rBSA, 0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Storage: 4 °C

Storage Comment:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Expiry Date: 24 months

Target Details for Melanoma gp100

Target: Melanoma gp100 (PMEL) (Premelanosome Protein (PMEL))

Alternative Name: gp100

Background:

Synonyms: 95 kDa melanocyte-specific secreted glycoprotein, M-beta, Melanocyte lineage specific antigen GP100, Melanocyte protein Pmel 17, Melanoma associated ME20 antigen, Melanosomal matrix protein17, p100, p26, PMEL17, Premelanosome protein, Secreted melanoma-associated ME20 antigen, SILV, Silver homolog

Gene Symbol: PMEL

Tissue Expression: Melanocytes

Molecular Weight: 90-100 kDa

Gene ID: 6490

UniProt: P40967