RPA2 antibody (CF®647)

Catalog No. ABIN7659551

The Mouse Monoclonal anti-RPA2 antibody has been validated for WB. It is suitable to detect RPA2 in samples from Human.

Quick Overview for RPA2 antibody (CF®647) (ABIN7659551)

Target: RPA2 (Replication Protein A2, 32kDa (RPA2))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This RPA2 antibody is conjugated to CF®647

Application: Western Blotting (WB)

Clone: RPA2-2106

Product Details anti-RPA2 Antibody (CF®647)

Purpose: Replication Protein A2 (RPA2)(RPA2/2106), CF647 conjugate

Characteristics: Recognizes a protein of 34 kDa, identified as a subunit of Replication Protein A (RPA) (also known as human single-stranded DNA binding protein, or HSSB). RPA from human cells is a stable heterotrimer of 70 kDa, 32-34 kDa, and 11-14 kDa subunits (RPA70, RPA32, and RPA14 respectively). It is involved in DNA replication, repair, and recombination. RPA is required for the SV40 large tumor antigen-catalyzed unwinding of SV40 DNA and stimulates DNA polymerase (pol) alpha and delta. RPA34 is phosphorylated at the G1/S boundary of the cell cycle or upon exposure of cells to DNA damage-inducing agents including ionizing and UV radiation. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant human full-length Replication protein A2 (RPA2) protein

Isotype: IgG2b, kappa

Application Details

Application Notes: Western blot 1-2 μg/mL, Optimal dilution for a specific application should be determined.

Comment:

Positive Control: MCF-7, HeLa or HepG2 cells. Human tonsil (IHC).

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % BSA, 0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Storage: 4 °C

Storage Comment:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Expiry Date: 24 months

Target Details for RPA2

Target: RPA2 (Replication Protein A2, 32kDa (RPA2))

Alternative Name: Replication Protein A2

Background:

Synonyms: p32, p34, REPA2, Replication protein A 32 kDa subunit, Replication protein A 34 kDa subunit, Replication protein A2, RF-A protein 2, Rf-A2, RFA, RP-A p32, RP-A p34, RP21C, RPA32, RPA34, RpLP1, RpP2

Gene Symbol: RPA2

Molecular Weight: 32-34 kDa

Gene ID: 6118

UniProt: P15927

Pathways: Telomere Maintenance, DNA Damage Repair, Mitotic G1-G1/S Phases, DNA Replication, Synthesis of DNA