STAR antibody (AA 39-108) (CF®740)

Catalog No. ABIN7660408

This Mouse Monoclonal antibody specifically detects STAR in IHC and IHC (fp). It exhibits reactivity toward Human.

Quick Overview for STAR antibody (AA 39-108) (CF®740) (ABIN7660408)

Target: STAR (Steroidogenic Acute Regulatory Protein (STAR))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This STAR antibody is conjugated to CF®740

Application: Immunohistochemistry (IHC), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Clone: STAR-2154

Product Details anti-STAR Antibody (CF®740)

Binding Specificity: AA 39-108

Purpose: StAR (Steroidogenic Acute Regulator) (Leydig Cell Marker) (STAR/2154), CF740 conjugate

Characteristics: Steroidogenic Acute Regulatory Protein (STAR) controls the rate-limiting step of steroidegenesis by translocating cholesterol from the outer mitochondrial membrane to the inner membrane where it is later cleaved to pregnenolone. It is primarily present in steroid-producing cells, including Leydig cells in the testis, theca cells and luteal cells in the ovary and adrenal cells in the adrenal cortex. Due to low levels of pregnenolone, seminomas and Leydig cell tumors display no specific STAR staining. Therefore, STAR antibody may assist in differentiating sex cord stromal tumors (SCST), seminomas and embryonal carcinomas.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant fragment (around aa 39-108) of human STAR protein

Isotype: IgG1, kappa

Application Details

Application Notes: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Immunohistology (formalin): 1-2 μg/mL for 30 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined by user

Comment:

Positive Control: K-562 cells. Adrenal or Testicular Carcinoma

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % rBSA, 0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Storage: 4 °C

Storage Comment:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Expiry Date: 24 months

Target Details for STAR

Target: STAR (Steroidogenic Acute Regulatory Protein (STAR))

Alternative Name: StAR

Background:

Synonyms: Cholesterol trafficker, Luteinizing hormone induced protein, Mitochondrial steroid acute regulatory protein, StAR, StAR related lipid transfer (START) domain containing 1, StARD1, START domain-containing protein 1, Steroid acute regulatory protein, Steroidogenic acute regulator (STAR), Steroidogenic acute regulatory protein mitochondrial

Gene Symbol: STAR

Tissue Expression: Adrenal gland|Ovary|Testis

Molecular Weight: ~30 kDa

Gene ID: 6770

UniProt: P49675

Pathways: Metabolism of Steroid Hormones and Vitamin D, Response to Growth Hormone Stimulus, C21-Steroid Hormone Metabolic Process, Cellular Response to Molecule of Bacterial Origin, Carbohydrate Homeostasis