TIA1 antibody (AA 279-380) (CF®740)

Catalog No. ABIN7660832

The Mouse Monoclonal anti-TIA1 antibody has been validated for WB and IF. It is suitable to detect TIA1 in samples from Human.

Quick Overview for TIA1 antibody (AA 279-380) (CF®740) (ABIN7660832)

Target: TIA1 (TIA1 Cytotoxic Granule-Associated RNA Binding Protein (TIA1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This TIA1 antibody is conjugated to CF®740

Application: Western Blotting (WB), Immunofluorescence (IF)

Clone: TIA1-1313

Product Details anti-TIA1 Antibody (CF®740)

Binding Specificity: AA 279-380

Purpose: TIA1 (T-Cell-Restricted Intracellular Antigen-1) (TIA1/1313), CF740 conjugate

Characteristics: TIA-1 (T-cell intracytoplasmic antigen) is a cytoplasmic granule-associated protein expressed in lymphocytes processing cytolytic potential. TIA-1 is a member of an RNA-binding protein family and possesses nucleolytic activity against cytotoxic lymphocyte (CTL) target cells. It has been suggested that this protein may be involved in the induction of apoptosis as it preferentially recognizes poly(A) homopolymers and induces DNA fragmentation in CTL targets. The major granule-associated species is a 15 kDa protein thought to be derived from the carboxyl terminus of the 40 kDa product by proteolytic processing. TIA1 antibody labels cytotoxic T cells and natural killer cells (NK cells). It is also expressed in T-cell lymphoma, large granular lymphocyte (LGL) leukemia and hairy cell leukemia. TIA1 expression in T-cell malignancies may help in differentiating LGL leukemia (high expression) from T-cell lymphocytosis and other T-cell diseases (low expression). TIA1 may also be used to label tumor-infiltrating lymphocytes in the study of immune response to malignancies.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant human TIA1 fragment of 102 amino acid residues (aa279-380)

Isotype: IgG2b

Application Details

Application Notes: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Immunofluorescence: 1-2 μg/mL. Flow cytometry: 0.5-1 μg/million cells in 0.1 mL. Western blotting 1-2 μg/mL. Optimal dilution for a specific application should be determined by user

Comment:

Positive Control: HepG2, PC3 and HeLa cells. Tonsil.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % rBSA, 0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Storage: 4 °C

Storage Comment:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Expiry Date: 24 months

Target Details for TIA1

Target: TIA1 (TIA1 Cytotoxic Granule-Associated RNA Binding Protein (TIA1))

Alternative Name: TIA1

Background:

Synonyms: Cytotoxic granule associated RNA binding protein 1, mTIA-1, Nucleolysin TIA1 isoform p40, p40-TIA-1, RNA binding protein TIA1, T-cell-restricted intracellular antigen-1 (TIA1), TIA-1, TIA1, TIA1 cytotoxic granule associated RNA binding protein, TIAL1, TIAR, WDM

Gene Symbol: TIA1

Molecular Weight: 40 kDa

Gene ID: 7072

UniProt: P31483