TOX3 antibody (AA 200-400) (CF®740)

Catalog No. ABIN7660931

The Mouse Monoclonal anti-TOX3 antibody is suitable to detect TOX3 in samples from Human. It has been validated for WB.

Quick Overview for TOX3 antibody (AA 200-400) (CF®740) (ABIN7660931)

Target: TOX3 (TOX High Mobility Group Box Family Member 3 (TOX3))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This TOX3 antibody is conjugated to CF®740

Application: Western Blotting (WB)

Clone: TOX3-1123

Product Details anti-TOX3 Antibody (CF®740)

Binding Specificity: AA 200-400

Purpose: TOX3(TOX3/1123), CF740 conjugate

Characteristics: This antibody recognizes a 63 kDa protein, which is identified as TOX3. It contains a high mobility group (HMG)-box, which regulates calcium-dependent transcription in neurons through interaction with the cAMP-response-element-binding protein (CREB). TOX3 appears to be associated with breast cancer susceptibility and is expressed downstream of a cytoprotective cascade together with CITED1, a transcriptional regulator that does not bind directly to DNA. TOX3 is predominantly expressed in the brain and forms homodimers. TOX3 overexpression protects neuronal cells from cell death caused by endoplasmic reticulum stress or BAX overexpression through the induction of anti-apoptotic transcripts and repression of pro-apoptotic transcripts. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant fragment (139 Amino acid residues around aa 200-400) of human TOX3 protein

Isotype: IgG2b, kappa

Application Details

Application Notes: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Immunofluorescence: 0.5-1 μg/mL. Immunohistology formalin-fixed 0.5-1 μg/mL. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0 or 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes. Flow Cytometry 0.5-1 μg/million cells/0.1 mL. Optimal dilution for a specific application should be determined by user

Comment:

Positive Control: SH-SY5Y, MDA-MB-435 cells. Brain or breast or gastric Carcinoma.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % rBSA, 0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Storage: 4 °C

Storage Comment:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Expiry Date: 24 months

Target Details for TOX3

Target: TOX3 (TOX High Mobility Group Box Family Member 3 (TOX3))

Alternative Name: TOX3

Background:

Synonyms: TOX3, CAG trinucleotide repeat-containing gene F9 protein, CAGF9, TOX high mobility group box family member 3, Trinucleotide repeat containing 9 (TNRC9)

Gene Symbol: TOX3

Molecular Weight: 63 kDa

Gene ID: 27324

UniProt: O15405

Pathways: Chromatin Binding