UCHL1 antibody (CF®740)

Catalog No. ABIN7661603

The Mouse Monoclonal anti-UCHL1 antibody has been validated for WB and IF. It is suitable to detect UCHL1 in samples from Human, Rat, Mouse, Cow, Pig, Guinea Pig, Rabbit, Dog, Zebrafish (Danio rerio) and Sheep.

Quick Overview for UCHL1 antibody (CF®740) (ABIN7661603)

Target: UCHL1 (Ubiquitin Carboxyl-terminal Esterase L1 (Ubiquitin Thiolesterase) (UCHL1))

Reactivity: Human, Rat, Mouse, Cow, Pig, Guinea Pig, Rabbit, Dog, Zebrafish (Danio rerio), Sheep

Host: Mouse

Clonality: Monoclonal

Conjugate: This UCHL1 antibody is conjugated to CF®740

Application: Western Blotting (WB), Immunofluorescence (IF)

Clone: 13C4

Product Details anti-UCHL1 Antibody (CF®740)

Purpose: pgp9.5 (UCHL-1)(13C4), CF740 conjugate

Characteristics: This MAb reacts with a protein of 20-30 kDa, identified as PGP9.5, also known as ubiquitin carboxyl-terminal hydrolase-1 (UchL1). Initially, PGP9.5 expression in normal tissues was reported in neurons and neuroendocrine cells but later it was found in distal renal tubular epithelium, spermatogonia, Leydig cells, oocytes, melanocytes, prostatic secretory epithelium, ejaculatory duct cells, epididymis, mammary epithelial cells, Merkel cells, and dermal fibroblasts. Furthermore, immunostaining for PGP9.5 has been shown in a wide variety of mesenchymal neoplasms as well. A mutation in PGP9.5 gene is believed to cause a form of Parkinson's disease. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Native UchL1 (PGP9.5) protein from brain

Isotype: IgG2a, kappa

Application Details

Application Notes: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Immunofluorescence: 1-2 μg/mL. Western blotting 0.5-1 μg/mL. Optimal dilution for a specific application should be determined by user

Comment:

Positive Control: Cerebellum

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % rBSA, 0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Storage: 4 °C

Storage Comment:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Expiry Date: 24 months

Target Details for UCHL1

Target: UCHL1 (Ubiquitin Carboxyl-terminal Esterase L1 (Ubiquitin Thiolesterase) (UCHL1))

Alternative Name: PGP9.5

Background:

Synonyms: Gracile Axonal Dystrophy|Neuron Cytoplasmic Protein 9.5|Park5|Parkinson Disease 5|PGP95|Protein Gene Product 9.5|Ubiquitin Carboxyl-terminal Esterase L1|Ubiquitin Carboxyl-terminal Hydrolase Isozyme L1|Ubiquitin Thioesterase L1|Ubiquitin Thiolesterase L1

Gene Symbol: UCHL1

Tissue Expression: Neurons

Molecular Weight: 20-30 kDa

Gene ID: 7345

UniProt: P09936

Pathways: Feeding Behaviour