ERBB4 antibody (AA 1116-1269)

Catalog No. ABIN7661752

This Mouse Monoclonal antibody specifically detects ERBB4 in IF and FACS. It exhibits reactivity toward Human and Mouse.

Quick Overview for ERBB4 antibody (AA 1116-1269) (ABIN7661752)

Target: ERBB4 (V-Erb-A erythroblastic Leukemia Viral Oncogene Homolog 4 (Avian) (ERBB4))

Reactivity: Human, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This ERBB4 antibody is un-conjugated

Application: Immunofluorescence (IF), Flow Cytometry (FACS)

Clone: HFR-1

Product Details anti-ERBB4 Antibody

Binding Specificity: AA 1116-1269

Purpose: HER-4 / ERBB4(HFR-1)

Characteristics: The EGF receptor family comprises several related receptor tyrosine kinases that are frequently overexpressed in a variety of carcinomas. Members of this receptor family include EGFR (HER1), Neu (ErbB-2, HER2), ErbB-3 (HER3) and ErbB-4 (HER4), which form either homodimers or heterodimers upon ligand binding. The gene encoding ErbB-4 is expressed as a full-length protein, which produces a short membrane-anchored cytoplasmic domain fragment and a long ectodomain fragment. The short fragment is heavily tyrosine phosphorylated and possesses tyrosine kinase catalytic activity toward an exogenous substrate. Proteolytic cleavage of ErbB-4 is promoted by the binding of heregulin. ErbB-4 is involved in cell proliferation and differentiation and its expression is highest in breast carcinoma cell lines, normal skeletal muscle, heart, pituitary, brain and cerebellum. Its expression in breast cancer, pediatric brain cancer and other types of carcinomas has been reported in studies which suggest ErbB4 expression is involved in both normal tissue development and carcinogenesis. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant human ERBB4 (HER4) protein fragment (around aa 1116-1269)

Isotype: IgG2b, kappa

Application Details

Application Notes: Predicted to react with Rat, Chicken, Flow cytometry: 1-2 μg/million cells, Immunofluorescence: 1-2 μg/mL, Optimal dilution for a specific application should be determined.

Comment:

Positive Control: Breast, brain, heart or kidney. MCF-7 cells.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: PBS, 0.05 % BSA, 0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment:

Stable at room temperature or 37°C for 7 days.

Store at 2 to 8°C. Protect fluorescent conjugates from light

Expiry Date: 24 months

Target Details for ERBB4

Target: ERBB4 (V-Erb-A erythroblastic Leukemia Viral Oncogene Homolog 4 (Avian) (ERBB4))

Alternative Name: ERBB4

Background:

Synonyms: Avian erythroblastic leukemia viral oncogene homolog 4, ERBB4, HER4, human epidermal growth factor receptor 4, p180erbB4, Proto-oncogene-like protein c-ErbB-4

Gene Symbol: ERBB4

Molecular Weight: 180 kDa (precursor), 80/120 kDa (cleaved)

Gene ID: 2066

UniProt: Q15303

Pathways: RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway