ERBB4 antibody (AA 1116-1269)

Catalog No. ABIN6939332

This Mouse Monoclonal antibody specifically detects ERBB4 in IF and FACS. It exhibits reactivity toward Human and Mouse.

Quick Overview for ERBB4 antibody (AA 1116-1269) (ABIN6939332)

Target: ERBB4 (V-Erb-A erythroblastic Leukemia Viral Oncogene Homolog 4 (Avian) (ERBB4))

Reactivity: Human, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This ERBB4 antibody is un-conjugated

Application: Immunofluorescence (IF), Flow Cytometry (FACS)

Clone: HFR-1

Product Details anti-ERBB4 Antibody

Binding Specificity: AA 1116-1269

Purpose: HER-4 / ERBB4 Antibody

Specificity: The EGF receptor family comprises several related receptor tyrosine kinases that are frequently overexpressed in a variety of carcinomas. Members of this receptor family include EGFR (HER1), Neu (ErbB-2, HER2), ErbB-3 (HER3) and ErbB-4 (HER4), which form either homodimers or heterodimers upon ligand binding. The gene encoding ErbB-4 is expressed as a full-length protein, which produces a short membrane-anchored cytoplasmic domain fragment and a long ectodomain fragment. The short fragment is heavily tyrosine phosphorylated and possesses tyrosine kinase catalytic activity toward an exogenous substrate. Proteolytic cleavage of ErbB-4 is promoted by the binding of heregulin. ErbB-4 is involved in cell proliferation and differentiation and its expression is highest in breast carcinoma cell lines, normal skeletal muscle, heart, pituitary, brain and cerebellum. Its expression in breast cancer, pediatric brain cancer and other types of carcinomas has been reported in studies which suggest ErbB4 expression is involved in both normal tissue development and carcinogenesis.

Cross-Reactivity (Details): Predicted to work with Rat, Chicken.

Purification: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Recombinant human ERBB4 (HER4) protein fragment (around aa 1116-1269) (exact sequence is proprietary)

Isotype: IgG2b, kappa

Application Details

Application Notes:

Positive Control: Breast, brain, heart or kidney. MCF-7 cells.

Known Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL)Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for ERBB4

Target: ERBB4 (V-Erb-A erythroblastic Leukemia Viral Oncogene Homolog 4 (Avian) (ERBB4))

Alternative Name: ERBB4

Background:

Avian erythroblastic leukemia viral oncogene homolog 4, ERBB4, HER4, human epidermal growth factor receptor 4, p180erbB4, Proto-oncogene-like protein c-ErbB-4

Brain, Breast, heart or kidney. MCF-7 cells.

Molecular Weight: 180kDa (precursor), 80/120kDa (cleaved)

Gene ID: 2066, 390729

UniProt: Q15303

Pathways: RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway