Vimentin antibody (AA 2-466) (CF®594)

Catalog No. ABIN7661942

The Mouse Monoclonal anti-Vimentin antibody is suitable to detect Vimentin in samples from Human. It has been validated for WB, IHC and IHC (fp).

Quick Overview for Vimentin antibody (AA 2-466) (CF®594) (ABIN7661942)

Target: Vimentin (VIM)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Vimentin antibody is conjugated to CF®594

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Clone: VIM-3736

Product Details anti-Vimentin Antibody (CF®594)

Binding Specificity: AA 2-466

Purpose: Vimentin (Mesenchymal Cell Marker)(VIM/3736), CF594 conjugate

Characteristics: This MAb reacts with a 58 kDa protein identified as vimentin. It shows no cross-reaction with other closely related intermediate filament proteins (IFP s) such as desmin, keratin, neurofilament, and glial fibrillary acid protein. Anti-vimentin alone is of limited value as a diagnostic tool, however, when used in panels with other antibodies, it is useful for the sub-classification of a given tumor. Expression of vimentin, when used in conjunction with anti-keratin, is helpful when distinguishing melanomas from undifferentiated carcinomas and large cell lymphomas. All melanomas and Schwannomas react strongly with anti-vimentin. It labels a variety of mesenchymal cells, including melanocytes, lymphocytes, endothelial cells, and fibroblasts. Non-reactivity of anti-vimentin is often considered more useful than its positive reactivity, since there are a few tumors that do not contain vimentin, e. g. hepatoma and seminoma. Anti-vimentin is also useful as a tissue process control reagent. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: A recombinant fragment (around aa 2-466) of human Vimentin protein

Isotype: IgG2b, kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Positive Control: U87, A375, Raji, HEP2 cells. Sarcomas or Melanomas.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % BSA, 0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Storage: 4 °C

Storage Comment:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Expiry Date: 24 months

Target Details for Vimentin

Target: Vimentin (VIM)

Alternative Name: Vimentin

Background:

Synonyms: VIM

Gene Symbol: VIM

Molecular Weight: 57-60 kDa

Gene ID: 7431

UniProt: P08670

Pathways: Caspase Cascade in Apoptosis