Cyclin F antibody (AA 451-550) (HRP)

Catalog No. ABIN732977

The Rabbit Polyclonal anti-Cyclin F antibody has been validated for WB, ELISA, IHC (p) and IHC (fro). It is suitable to detect Cyclin F in samples from Human and Rat.

Quick Overview for Cyclin F antibody (AA 451-550) (HRP) (ABIN732977)

Target: Cyclin F (CCNF)

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Cyclin F antibody is conjugated to HRP

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-Cyclin F Antibody (HRP)

Binding Specificity: AA 451-550

Cross-Reactivity: Human, Rat

Predicted Reactivity: Mouse,Dog,Cow,Pig,Horse,Chicken,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human Cyclin F

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
IHC-P 1:200-400
IHC-F 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handling Advice: Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for Cyclin F

Target: Cyclin F (CCNF)

Alternative Name: Cyclin F

Background:

Synonyms: FBX1, FBXO1, Cyclin-F, F-box only protein 1, CCNF

Background: Substrate recognition component of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of CP110 during G2 phase, thereby acting as an inhibitor of centrosome reduplication.

Gene ID: 899

UniProt: P41002