CD45RO antibody

Catalog No. ABIN733283

The Rabbit Polyclonal anti-CD45RO antibody has been validated for IHC (p), WB and IF (p). It is suitable to detect CD45RO in samples from Human, Mouse and Rat.

Quick Overview for CD45RO antibody (ABIN733283)

Target: CD45RO

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CD45RO antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Product Details anti-CD45RO Antibody

Cross-Reactivity: Human

Purification: Purified by Protein A.

Immunogen: Cultured T cells from human IL-2-dependent T-cell line (CA1) prepared from human peripheral blood activated with influenza virus.

Isotype: IgG

Application Details

Application Notes: WB(1:100-500)
Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 1 % BSA, 50 % glycerol and 0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C for 12 months.

Expiry Date: 12 months

Target Details for CD45RO

Target: CD45RO

Alternative Name: CD45RO

Background:

Synonyms: B220, CD 45, CD45, cd45 antigen, ec3.1.3.48, CD45R, GP180, GP180, GP 180, L CA, LCA, L-CA, Leukocyte common antigen, LY5, Protein tyrosine phosphatase receptor type C, Protein tyrosine phosphatase receptor type c polypeptide, protein tyrosine phosphatase, receptor type, C, Receptor-type tyrosine-protein phosphatase C, PTPRC, PTPRC_HUMAN, SCID due to PTPRC deficiency, T200, T200 glycoprotein, T200 leukocyte common antigen, Human homolog of severe combined immunodeficiency due to PTPRC deficiency.

Background: CD45RO is a single chain transmembraneous glycoprotein which represents the low molecular weight isoform of the Leucocyte Common Antigen (LCA). It is expressed on most thymocytes, about 45 % of peripheral blood T cells, virtually all T cells in skin reactive infiltrates, and the majority of T cell malignancies. It is also found on a subset of B cells and on exceptional B cell lymphomas.CD45 is a family of single chain transmembraneous glycoproteins consisting of at least four isoforms (220, 205, 190, 180 kDa) which share a common large intracellular domain. Their extracellular domains are heavily glycosylated. The different isoforms are produced by alternative messenger RNA splicing of three exons of a single gene on chromosome 1. CD45 is expressed on cells of the human hematopoietic lineage (including hematopoietic stem cells) with the exception of mature red cells. It is not detected on differentiated cells of other tissues. It is likely that CD45 plays an important role in signal transduction, inhibition or upregulation of various immunological functions. recognising a common epitope on all of the isoforms are termed CD45 whilst those recognising only individual isoforms are termed CD45RA or CD45RO etc.

Molecular Weight: 180kDa

Gene ID: 5788