Recombinant His Tag antibody
The Rabbit Monoclonal anti-His Tag antibody has been validated for WB, ELISA, IP and ICC. It is suitable to detect His Tag in samples from .
Quick Overview for Recombinant His Tag antibody (ABIN7670359)
Target
See all His Tag Antibodies
His Tag
Antibody Type
Recombinant Antibody
Reactivity
Please inquire
Host
Rabbit
Clonality
Monoclonal
Conjugate
This His Tag antibody is un-conjugated
Application
Western Blotting (WB), ELISA, Immunoprecipitation (IP), Immunocytochemistry (ICC)
Clone
RM146X
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Purpose
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Rabbit monoclonal to His-Tag at either the N-Terminus (amino) or C-Terminus (carboxy)
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Specificity
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This antibody reacts to recombinant proteins containing the His-Tag fused to either the amino or carboxy terminus.
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Cross-Reactivity (Details)
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Very low non-specific reactivity with endogenous proteins in mammalian or bacteria cells, compared with other anti-HisTag antibodies.
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Purification
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Protein A affinity purified from an animal origin-free culture supernatant
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Immunogen
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6xHis-Tag coupled to KLH
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Isotype
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IgG
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Application Notes
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WB: 0.01 μg/mL - 0.1 μg/mL, ELISA: 0.01 μg/mL - 0.2 μg/mL, IP: 0.5 μg/mL - 2 μg/mL, ICC: 0.5 μg/mL - 2 μg/mL.
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Restrictions
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For Research Use only
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Format
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Liquid
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Concentration
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1 mg/mL
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Buffer
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50 % Glycerol/PBS with 1 % BSA and 0.09 % sodium azide
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Preservative
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Sodium azide
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Precaution of Use
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This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Storage
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-20 °C
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Storage Comment
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-20.0°C
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Target
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His Tag
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Alternative Name
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His-Tag
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Target Type
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Tag
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