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Recombinant His Tag antibody

The Rabbit Monoclonal anti-His Tag antibody has been validated for WB, ELISA, IP and ICC. It is suitable to detect His Tag in samples from .
Catalog No. ABIN7670359

Quick Overview for Recombinant His Tag antibody (ABIN7670359)

Target

See all His Tag Antibodies
His Tag

Antibody Type

Recombinant Antibody

Reactivity

Please inquire

Host

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Rabbit

Clonality

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Monoclonal

Conjugate

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This His Tag antibody is un-conjugated

Application

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Western Blotting (WB), ELISA, Immunoprecipitation (IP), Immunocytochemistry (ICC)

Clone

RM146X
  • Purpose

    Rabbit monoclonal to His-Tag at either the N-Terminus (amino) or C-Terminus (carboxy)

    Specificity

    This antibody reacts to recombinant proteins containing the His-Tag fused to either the amino or carboxy terminus.

    Cross-Reactivity (Details)

    Very low non-specific reactivity with endogenous proteins in mammalian or bacteria cells, compared with other anti-HisTag antibodies.

    Purification

    Protein A affinity purified from an animal origin-free culture supernatant

    Immunogen

    6xHis-Tag coupled to KLH

    Isotype

    IgG
  • Application Notes

    WB: 0.01 μg/mL - 0.1 μg/mL, ELISA: 0.01 μg/mL - 0.2 μg/mL, IP: 0.5 μg/mL - 2 μg/mL, ICC: 0.5 μg/mL - 2 μg/mL.

    Restrictions

    For Research Use only
  • Format

    Liquid

    Concentration

    1 mg/mL

    Buffer

    50 % Glycerol/PBS with 1 % BSA and 0.09 % sodium azide

    Preservative

    Sodium azide

    Precaution of Use

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Storage

    -20 °C

    Storage Comment

    -20.0°C
  • Target

    His Tag

    Alternative Name

    His-Tag

    Target Type

    Tag
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