Endoglin antibody

Catalog No. ABIN7670510

This Rabbit Polyclonal antibody specifically detects Endoglin in WB. It exhibits reactivity toward Mouse.

Quick Overview for Endoglin antibody (ABIN7670510)

Target: Endoglin (ENG)

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Endoglin antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-Endoglin Antibody

Purpose: CD105 Polyclonal Antibody

Purification: Antigen Affinity Purification

Immunogen: Recombinant Mouse CD105 protein expressed by Mammalian

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:1000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS with 0.05 % proclin 300, 1 % protective protein and 50 % glycerol, pH 7.4

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.

Expiry Date: 12 months

Target Details for Endoglin

Target: Endoglin (ENG)

Alternative Name: CD105

Background: END,HHT1,ORW,ORW1,CD 105,CD105 antigen,EGLN,FLJ41744,Osler Rendu Weber syndrome 1,RP11 228B15.2,SN6,HHT,ORW1,AI528660,AI662476,CD105,END,Endo,Endoglin,HHT1,ORW,S-endoglin,Edg,Eng,Endoglin (CD105) is a 90 kDa type I transmembrane glycoprotein of the zona pellucida (ZP) family of proteins. Endoglin and betaglycan/T beta RIII are type III receptors for TGF beta superfamily ligands,sharing 71 % aa identity in the transmembrane (TM) and cytoplasmic domains. Endoglin is highly expressed on proliferating vascular endothelial cells,chondrocytes,and syncytiotrophoblasts of term placenta,with lower amounts on hematopoietic,mesenchymal and neural crest stem cells,activated monocytes,and lymphoid and myeloid leukemic cells. Human endoglin cDNA encodes 658 amino acids (aa) including a 25 aa signal sequence,a 561 aa extracellular domain (ECD) with an orphan domain and a two-part ZP domain,a TM domain and a 47 aa cytoplasmic domain. An isoform with a 14 aa cytoplasmic domain (S-endoglin) can oppose effects of long (l) endoglin. The human endoglin ECD shares 65-72 % aa identity with mouse,rat,bovine,porcine and canine endoglin. Endoglin homodimers interact with TGF-beta 1 and TGF-beta 3 (but not TGF-beta 2),but only after binding T beta RII. Similarly,they interact with Activin A and BMP-7 via activin type IIA or B receptors,and with BMP-2 via BMPR-IA/AlK-3 or BMPR-IB/AlK-6. BMP-9,however,is reported to bind endoglin directly. Endoglin modifies ligand-induced signaling in multiple ways. For example,expression of endoglin can inhibit TGF-beta 1 signals but enhance BMP-7 signals in the same myoblast cell line.

Molecular Weight:

Calculated MW: 71 kDa

ObservedMW: 95-100 kDa

UniProt: Q63961