HMGB1 antibody

Catalog No. ABIN7670629

This anti-HMGB1 antibody is a Rabbit Polyclonal antibody detecting HMGB1 in WB and ELISA. Suitable for Human, Mouse and Rat.

Quick Overview for HMGB1 antibody (ABIN7670629)

Target: HMGB1 (High Mobility Group Box 1 (HMGB1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HMGB1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-HMGB1 Antibody

Purpose: HMGB-1 Polyclonal Antibody(Capture/Detector)

Purification: Antigen Affinity Purification

Immunogen: Recombinant Human HMGB-1 protein expressed by E.coli

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:1000,ELISA Capture 2-8 μg/mL,ELISA Detector 0.1-0.4 μg/mL

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: Sterile PBS , pH 7.4

Preservative: Without preservative

Handling Advice: Avoid freeze / thaw cycles. This preparation contains no preservatives, thus it should be handled under aseptic conditions.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C Valid for 1 month or -20°C Valid for 12 months

Expiry Date: 12 months

Target Details for HMGB1

Target: HMGB1 (High Mobility Group Box 1 (HMGB1))

Alternative Name: HMGB-1

Background: HMGB1,HMG1,High mobiLity group protein B1,High mobiLity group protein 1 (HMG-1),Human High-mobility group box 1 protein (HMGB1),previously known as HMG-1 or amphoterin,is a member of the high mobility group box family of non-histone chromosomal proteins.HMGB1 is expressed at high levels in almost all cells. It was originally discovered as a nuclear protein that could bend DNA. Such bending stabilizes nucleosome formation and regulates the expression of select genes upon recruitment by DNA binding proteins. It is now known that HMGB1 can also act extracellularly,both as an inflammatory mediator that promotes monocyte migration and cytokine secretion,and as a mediator of T cell-dendritic cell interaction. The cytokine activity of HBMG1 is restricted to the HMG B box,while the A box is associated with the helix-loop-helix domain of transcription factors. HMBG1 is released in response to cell death and as a secretion product. Although HMBG-1 does not possess a classic signal sequence,it appears to be secreted as an acetylated form via secretory endolysosome exocytosis. Once secreted,HMGB1 transduces cellular signals through its high affinity receptor,RAGE and,possibly,TlR2 and TlR4. Human HMGB1 is 100 % aa identical to canine HMGB1 and 99 % aa identical to mouse,rat,bovine and porcine HMGB1,respectively.

Molecular Weight:

Calculated MW: 29kDa

ObservedMW: 29kDa

UniProt: P09429

Pathways: p53 Signaling, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process, Toll-Like Receptors Cascades, Smooth Muscle Cell Migration, Inflammasome