Leptin antibody

Catalog No. ABIN7670678

This Rat Monoclonal antibody specifically detects Leptin in ELISA (Detection). It exhibits reactivity toward Human.

Quick Overview for Leptin antibody (ABIN7670678)

Target: Leptin (LEP)

Reactivity: Human

Host: Rat

Clonality: Monoclonal

Conjugate: This Leptin antibody is un-conjugated

Application: ELISA (Detection)

Clone: 7C1

Product Details anti-Leptin Antibody

Purpose: Leptin/OB Monoclonal Antibody(Detector)

Purification: Protein A/G Purification

Immunogen: Recombinant Human Leptin/OB protein expressed by E.coli

Isotype: IgG2a

Application Details

Application Notes: ELISA Detector 0.1-0.4 μg/mL

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: Sterile PBS , pH 7.4

Preservative: Without preservative

Handling Advice: Avoid freeze / thaw cycles. This preparation contains no preservatives, thus it should be handled under aseptic conditions.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C Valid for 1 month or -20°C Valid for 12 months

Expiry Date: 12 months

Target Details for Leptin

Target: Leptin (LEP)

Alternative Name: Leptin/OB

Background: LEP,Leptin,Obese protein,Obesity factor,OB,OBS,Key player in the regulation of energy balance and body weight control. Once released into the circulation,has central and peripheral effects by binding lEPR,found in many tissues,which results in the activation of several major signaling pathways.In the hypothalamus,acts as an appetite-regulating factor that induces a decrease in food intake and an increase in energy consumption by inducing anorexinogenic factors and suppressing orexigenic neuropeptides,also regulates bone mass and secretion of hypothalamo-pituitary-adrenal hormones. In the periphery,increases basal metabolism,influences reproductive function,regulates pancreatic beta-cell function and insulin secretion,is pro-angiogenic for endothelial cell and affects innate and adaptive immunity.In the arcuate nucleus of the hypothalamus,activates by depolarization POMC neurons inducing FOS and SOCS3 expression to release anorexigenic peptides and inhibits by hyperpolarization NPY neurons inducing SOCS3 with a consequent reduction on release of orexigenic peptides.In addition to its known satiety inducing effect,has a modulatory role in nutrient absorption. In the intestine,reduces glucose absorption by enterocytes by activating PKC and leading to a sequential activation of p38,PI3K and ERK signaling pathways which exerts an inhibitory effect on glucose absorption.Acts as a growth factor on certain tissues,through the activation of different signaling pathways increases expression of genes involved in cell cycle regulation such as CCND1,via JAK2-STAT3 pathway,or VEGFA,via MAPK1/3 and PI3K-AKT1 pathways. May also play an apoptotic role via JAK2-STAT3 pathway and up-regulation of BIRC5 expression.Pro-angiogenic,has mitogenic activity on vascular endothelial cells and plays a role in matrix remodeling by regulating the expression of matrix metalloproteinases (MMPs) and tissue inhibitors of metalloproteinases (TIMPs).In innate immunity,modulates the activity and function of neutrophils by increasing chemotaxis and the secretion of oxygen radicals. Increases phagocytosis by macrophages and enhances secretion of pro-inflammatory mediators. Increases cytotoxic ability of NK cells.

UniProt: P41159

Pathways: JAK-STAT Signaling, AMPK Signaling, Hormone Transport, Peptide Hormone Metabolism, Hormone Activity, Negative Regulation of Hormone Secretion, Regulation of Carbohydrate Metabolic Process, Feeding Behaviour, Monocarboxylic Acid Catabolic Process