Cytokeratin 7 antibody (AA 251-350)

Catalog No. ABIN733823

The Rabbit Polyclonal anti-Cytokeratin 7 antibody has been validated for WB, IHC (p), ELISA, IHC (fro), IF (cc) and IF (p). It is suitable to detect Cytokeratin 7 in samples from Human, Rat and Mouse. There is 1 publication available.

Quick Overview for Cytokeratin 7 antibody (AA 251-350) (ABIN733823)

Target: Cytokeratin 7 (KRT7) (Keratin 7 (KRT7))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Cytokeratin 7 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), ELISA, Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Product Details anti-Cytokeratin 7 Antibody

Binding Specificity: AA 251-350

Cross-Reactivity: Human, Mouse, Rat

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from mouse CK7

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References for anti-Cytokeratin 7 antibody (ABIN733823)

Zhang, Huang, Han, Hu, Yi, Yao, He: "Functional regeneration of irradiated salivary glands with human amniotic epithelial cells transplantation." in: International journal of clinical and experimental pathology, Vol. 6, Issue 10, pp. 2039-47, (2013) (PubMed).

Target Details for Cytokeratin 7

Target: Cytokeratin 7 (KRT7) (Keratin 7 (KRT7))

Alternative Name: CK7

Background:

Synonyms: K7, Krt2-7, D15Wsu77e, Keratin, type II cytoskeletal 7, Cytokeratin-7, CK-7, Keratin-7, Type-II keratin Kb7, Krt7

Background: Cytokeratins are a subfamily of intermediate filament proteins and are characterized by a remarkable biochemical diversity, represented in human epithelial tissues by at least 20 different polypeptides. They range in molecular weight between 40 kDa and 68 kDa and isoelectric pH between 4.9 7.8. The individual human cytokeratins are designated 1 to 20. The various epithelia in the human body usually express cytokeratins which are not only characteristic of the type of epithelium, but also related to the degree of maturation or differentiation within an epithelium. Cytokeratin subtype expression patterns are used to an increasing extent in the distinction of different types of epithelial malignancies. The cytokeratin are not only of assistance in the differential diagnosis of tumors using immunohistochemistry on tissue sections, but are also a useful tool in cytopathology and flow cytometric assays.

Gene ID: 110310

UniProt: Q9DCV7