CHEK1 antibody (pSer280)

Catalog No. ABIN7674368

This Rabbit Polyclonal antibody specifically detects CHEK1 in WB, ELISA, IHC and IF. It exhibits reactivity toward Human and Mouse.

Quick Overview for CHEK1 antibody (pSer280) (ABIN7674368)

Target: CHEK1 (Checkpoint Kinase 1 (CHEK1))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CHEK1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-CHEK1 Antibody

Binding Specificity: pSer280

Purpose: Phospho-CHEK1 antibody

Purification: Immunogen affinity purified

Purity: ≥95 % as determined by SDS-PAGE

Immunogen: Phospho-CHEK1-S280

Isotype: IgG

Application Details

Application Notes: WB: 1:500 - 1:2000, IHC: 1:50 - 1:200, IF: 1:50 - 1:200

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS with 0.02 % sodium azide and 50 % glycerol pH 7.3,

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze / thaw cycles.

Storage: -20 °C

Storage Comment: -20°C for 12 months (Avoid repeated freeze / thaw cycles.)

Expiry Date: 12 months

Target Details for CHEK1

Target: CHEK1 (Checkpoint Kinase 1 (CHEK1))

Alternative Name: CHEK1

Background:

Synonyms: Serine/threonine-protein kinase Chk1|CHK1 checkpoint homolog|Cell cycle checkpoint kinase|Checkpoint kinase-1|CHEK1|CHK1

Background: The protein encoded by this gene belongs to the Ser/Thr protein kinase family. It is required for checkpoint mediated cell cycle arrest in response to DNA damage or the presence of unreplicated DNA. This protein acts to integrate signals from ATM and ATR, two cell cycle proteins involved in DNA damage responses, that also associate with chromatin in meiotic prophase I. Phosphorylation of CDC25A protein phosphatase by this protein is required for cells to delay cell cycle progression in response to double-strand DNA breaks. Several alternatively spliced transcript variants have been found for this gene.

Molecular Weight: 60 kDa

Gene ID: 1111

UniProt: O14757

Pathways: p53 Signaling, Apoptosis, Cell Division Cycle, DNA Damage Repair