PKM2 antibody

Catalog No. ABIN7674413

This Rabbit Polyclonal antibody specifically detects PKM2 in WB, IHC, ELISA, IF and IP. It exhibits reactivity toward Human and Mouse.

Quick Overview for PKM2 antibody (ABIN7674413)

Target: PKM2 (Pyruvate Kinase M2 (PKM2))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PKM2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Immunoprecipitation (IP)

Product Details anti-PKM2 Antibody

Purpose: PKM2-specific antibody

Purification: Immunogen affinity purified

Purity: ≥95 % as determined by SDS-PAGE

Immunogen: PKM2 pyruvate kinase, muscle

Isotype: IgG

Application Details

Application Notes: WB: 1:1000-1:4000, IP: 1:200-1:1000, IHC: 1:100-1:400, IF: 1:10-1:100

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS with 0.02 % sodium azide and 50 % glycerol pH 7.3,

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze / thaw cycles.

Storage: -20 °C

Storage Comment: -20°C for 12 months (Avoid repeated freeze / thaw cycles.)

Expiry Date: 12 months

Target Details for PKM2

Target: PKM2 (Pyruvate Kinase M2 (PKM2))

Alternative Name: PKM2

Background:

Synonyms: Pyruvate kinase PKM|Cytosolic thyroid hormone-binding protein (CTHBP)|Opa-interacting protein 3 (OIP-3)|Pyruvate kinase 2/3|Pyruvate kinase muscle isozyme|Threonine-protein kinase PKM2|Thyroid hormone-binding protein 1 (THBP1)|Tumor M2-PK|Tyrosine-protein kinase PKM2|p58|PKM|OIP3|PK2|PK3|PKM2

Background: PKM2, also named as OIP3, PK2, PK3, PKM, p58, THBP1, CTHBP and Tumor M2-PK, belongs to the pyruvate kinase family. It is glycolytic enzyme that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate (PEP) to ADP, generating ATP. It stimulates POU5F1-mediated transcriptional activation. PKM2 plays a general role in caspase independent cell death of tumor cells. The activity of the M2 isoform (but not the M1 isoform) can be inhibited by tyrosine kinase signalling in tumourcells.The primary pyruvate kinase isoform before tumour development is PK-M1, however, the primary isoform from four independent tumours is PK-M2.(PMID:18337823) PKM2, Pyruvate kinase isozymes M1/M2, has 2 isoforms.The immunogen of this antibody is M2 isoform, also called PKM2. This antibody is specific to PKM2 isoform.

Molecular Weight: 58 kDa

UniProt: P14618

Pathways: Warburg Effect