Recombinant Vimentin antibody

Catalog No. ABIN7674794

This anti-Vimentin antibody is a Mouse Monoclonal antibody detecting Vimentin in WB, IHC, ICC, IHC (fro) and D. Suitable for Mouse.

Quick Overview for Recombinant Vimentin antibody (ABIN7674794)

Target: Vimentin (VIM)

Antibody Type: Recombinant Antibody

Reactivity: Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This Vimentin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Detection (D)

Clone: RV204

Product Details anti-Vimentin Antibody

Purpose: Recombinant mouse anti vimentin

Specificity: Human and mouse vimentin

Characteristics: Vimentin (57 kDa) is the intermediate filament protein (IFP) of mesenchymal cells. This IFP however often deviates from the tissue-specific and developmentally regulated pattern of expression. Besides its typical expression in most cultured cells, vimentin is also expressed together with several other IFPs during early stages of development. As differentiation proceeds, vimentin is exchanged for the tissue-specific intermediate filament type. Also in cancers, vimentin is often expressed in addition to the tissue-specific IFP.

Purification: ProtA purified

Immunogen: This clone RV204 is a recombinant mouse anti vimentin monoclonal antibody produced in mammalian HEK293 cells. Clone RV204 is produced by cloning the antibody sequence of the mouse anti vimentin hybridoma RV202, which was derived by fusion of SP2/0-Ag14 mouse myeloma cells with spleen cells from a BALB/c mouse immunized with a cytoskeletal vimentin extract of calf lens.

Isotype: IgG1

Application Details

Application Notes: This clone RV204 is suitable for immunoblotting, immunocytochemistry, and immunohistochemistry on frozen and paraffin-embedd tissue sections. Optimal antibody dilution should be determined by titration, recommended range is 1:100 - 1:200 for immunohistochemistry with fluorochrome conjugated secondary antibodies or with avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent, and 1:100 - 1:1000 for immunoblotting applications.

Restrictions: For Research Use only

Handling

Reconstitution: Each vial contains 100 µL 1 mg/mL ProtA purified recombinant monoclonal antibody in PBS containing 0.09 % sodium azide. The purity of the antibody is >98 % as determined by SDS-Polyacrylamide gel electrophoresis. The endotoxin level is < 1EU/mg as determined by the LAL chromogenic endotoxin assay.

Storage: 4 °C,-20 °C

Storage Comment: The antibody is shipped at ambient temperature and may be stored at +4°C. For prolonged storage prepare appropriate aliquots and store at or below -20°C. Prior to use, an aliquot is thawed slowly in the dark at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance or the concentration of the product.

Target Details for Vimentin

Target: Vimentin (VIM)

Alternative Name: vimentin

UniProt: P08670

Pathways: Caspase Cascade in Apoptosis