ITGA4 antibody (PE-FineTest®594)

Catalog No. ABIN7675140

This Mouse Monoclonal antibody specifically detects ITGA4 in FACS. It exhibits reactivity toward Human.

Quick Overview for ITGA4 antibody (PE-FineTest®594) (ABIN7675140)

Target: ITGA4 (Integrin alpha 4 (ITGA4))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This ITGA4 antibody is conjugated to PE-FineTest®594

Application: Flow Cytometry (FACS)

Clone: 9F10

Product Details anti-ITGA4 Antibody (PE-FineTest®594)

Purpose: PE-FineTest®594 Anti-Human CD49d Antibody

Isotype: IgG1, kappa

Application Details

Application Notes: Volume per test: 5μL. Each lot of this antibody is quality control tested by flow cytometric analysis. The amount of the reagent is suggested to be used 5 μL of antibody per test (million cells in 100 μL staining volume or per 100 μL of whole blood). Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use.

Comment:

PE-FineTest®594 is designed to be excited by the blue (488 nm), Green (532 nm) and yellow-green (561 nm) lasers and detected using an optical filter centered near 620 nm (e.g., a 610/20 nm bandpass filter).

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS with 0. 1 % sodium azide, 1 %BSA, pH 7.3,

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze / thaw cycles.

Storage: 4 °C

Storage Comment: 2-8°C for 12 months (Avoid repeated freeze / thaw cycles.)

Expiry Date: 12 months

Target Details for ITGA4

Target: ITGA4 (Integrin alpha 4 (ITGA4))

Alternative Name: CD49d

Background:

Synonyms: CD49 antigen-like family member D|CD49d|ITGA4|Integrin alpha-4|Integrin alpha-IV|VLA-4 subunit alpha

Background: CD49d is a 150 kD α integrin chain known as α4 integrin or VLA-4 α chain. It forms a heterodimer with either integrin β1 (α4β1, VLA-4) or β7 (α4β7). CD49d is expressed broadly on T lymphocytes, B lymphocytes, monocytes, thymocytes, eosinophils, basophils, mast cells, NK cells, dendritic cells, and some non-hematopoietic cells, but not on normal red blood cells, platelets or neutrophils. VLA-4 binds to VCAM-1 (CD106) and fibronectin. α4β7 is the receptor for VCAM-1 and MAdCAM-1. CD49d participates in mononuclear cell trafficking to endothelial sites of inflammation and has roles in cell-cell interactions and cell adhesion to extracellular matrices. CD49d is involved in lymphocyte migration, T cell activation, and hematopoietic stem cell differentiation. CD49d is a marker to isolate pure populations of Treg cells due to its absence on Foxp3+ cells.

Molecular Weight: 0

Gene ID: 3676

UniProt: P13612

Pathways: Integrin Complex